Involvement of protein kinase A and a kinase anchoring protein in the progesterone-initiated human sperm acrosome reaction

被引:65
作者
Harrison, DA
Carr, DW
Meizel, S
机构
[1] Univ Calif Davis, Dept Cell Biol & Human Anat, Sch Med, Davis, CA 95616 USA
[2] Vet Affairs Med Ctr, Portland, OR 97201 USA
[3] Oregon Hlth & Sci Univ, Portland, OR 97201 USA
关键词
D O I
10.1095/biolreprod62.3.811
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The signal transduction pathways involved in the progesterone (P-4)-initiated mammalian sperm acrosome reaction (AR) are not fully understood. To investigate the role of the protein kinase A (PKA) pathway in the P-4-initiated AR, we probed this pathway by pretreating capacitated human sperm with reagents designed to either inhibit PKA activation or disrupt PKA/A kinase anchoring protein (AKAP) interactions. Preincubation with the stearated (membrane permeable) PKA inhibitor, PKI alpha 5-24 (S-PKI alpha 5-24), significantly inhibited the P-4-initiated AR at 10 mu M as compared to stearated control peptide. In contrast, preincubation with 100 mu M nonstearated PKI alpha 5-24 did not significantly inhibit versus solvent control. Preincubation with the PKA inhibitor Rp-8-Br-cAMP at 500 mu M and 150 mu M significantly inhibited the P-4-initiated AR versus 8-Br-cAMP and versus solvent. Preincubation with the anchoring inhibitory peptide S-Ht31 significantly stimulated the P-4-initiated AR at 10, 3, and 1 mu M versus inactive control peptide, The stimulation of the P-4-initiated AR by 3 mu M S-Ht31 was significantly inhibited by the addition of 30 mu M S-PKI alpha 5-24 prior to the addition of S-Ht31. Preincubation with S-PKI alpha 5-24 (30 mu M) partially inhibited the ionomycin (50 mu M)-initiated AR. A role for PKA in the P-4-initiated AR may exist both upstream and downstream of Ca2+ entry. Our studies present the first evidence for the participation of PKA in the P-4-initiated AR and also suggest that AKAPs are involved in the PKA-mediated events.
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页码:811 / 820
页数:10
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