Evaluation of soil bacterial biomass using environmental DNA extracted by slow-stirring method

被引：26

作者：

Aoshima, H. ^{[1
]}

Kimura, A. ^{[1
]}

Shibutani, A. ^{[1
]}

Okada, C. ^{[1
]}

Matsumiya, Y. ^{[1
]}

Kubo, M. ^{[1
]}

机构：

[1] Ritsumeikan Univ, Fac Sci & Engn, Dept Biosci & Technol, Shiga 5258577, Japan

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2006年 / 71卷 / 06期

关键词：

D O I：

10.1007/s00253-005-0245-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple and rapid method (slow-stirring method) for extracting environmental DNA (eDNA) from soils was constructed by physical mild stirring with chemical treatment. eDNA was extracted efficiently with minimal damage from various kinds of soil. The amount of eDNA and soil bacterial biomass showed a linear proportional relation [Y=(1.70x10(8))X, r(2)=0.96], indicating that bacterial biomass could be evaluated by quantifying levels of eDNA. Consequently, the average bacterial biomass in an agricultural field was calculated as 5.95x10(9) cells/g sample, approximately 10-100 times higher than that in non- and oil-polluted fields.

引用

页码：875 / 880

页数：6

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