Isolation and characterization of subgenomic DNAs encapsidated in "single" T=1 isometric particles of Maize streak virus

被引：32

作者：

Casado, CG

Ortiz, GJ

Padron, E

Bean, SJ

McKenna, R

Agbandje-McKenna, M ^{[1
]}

Boulton, MI

机构：

[1] Univ Florida, Coll Med, McKnight Brain Inst, Dept Biochem & Mol Biol, Gainesville, FL 32610 USA

[2] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England

[3] John Innes Ctr Plant Sci Res, Dept Dis & Stress Biol, Norwich NR4 7UH, Norfolk, England

来源：

VIROLOGY | 2004年 / 323卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 美国国家科学基金会;

关键词：

Maize streak virus; subgenomic DNAs; isometric particles; Geminivirus; coat protein;

D O I：

10.1016/j.virol.2004.02.014

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Single T = 1 isometric particles of Maize streak virus (MSV) have been isolated from infected maize leaves. Biochemical and genetic characterizations show that these particles contain subgenomic (sg) MSV DNA encapsidated by the MSV coat protein. The largest sg DNA is 1.56 kb, slightly larger than half genome size, although sg DNAs as small as 0.2 kb were also cloned. The sg DNAs are not infectious, and they do not appear to play a role in the pathogenicity of MSV. This is the first report of sg DNAs for MSV and, to our knowledge, the first time that encapsidated sg DNAs have been characterized at the sequence level for any geminivirus. These data will assist in our investigations into the role of genomic DNA in the formation of the unique geminate capsid architecture of the Geminiviridae. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：164 / 171

页数：8

共 33 条

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