Anti-oxidant enzymes in Cryptosporidium parvum oocysts

被引：22

作者：

Entrala, E

Mascaro, C

Barrett, J

机构：

[1] UNIV WALES,INST BIOL SCI,ABERYSTWYTH SY23 3DA,DYFED,WALES

[2] UNIV GRANADA,FAC CIENCIAS,DEPT PARASITOL,E-18071 GRANADA,SPAIN

来源：

PARASITOLOGY | 1997年 / 114卷

关键词：

Cryptosporidium parvum; anti-oxidant enzymes; SOD;

D O I：

10.1017/S0031182096008037

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Oocysts of Cryptosporidium parvum showed relatively low levels of SOD activity. The SOD which had a pI of 4 . 8 and an approximate molecular weight of 35 kDa appeared to be iron dependent. Catalase, glutathione transferase, glutathione reductase and glutathione peroxidase activity could not be detected, nor could trypanothione reductase. No NADH or NADPH oxidase activity could be detected, nor could peroxidase activity be demonstrated using o-dianisidine, guaiacol, NADPH or NADH as co-substrates. However, an NADPH-dependent H2O2 scavenging system was detected in the insoluble fraction.

引用

页码：13 / 17

页数：5

共 23 条

[1] ENDOGENOUS SUPEROXIDE-DISMUTASE ACTIVITY IN 2 BABESIA SPECIES [J].