High-Resolution Structural Analysis of a DNA Nanostructure by cryoEM

被引：68

作者：

Kato, Takayuki ^{[1
]}

Goodman, Russell P. ^{[3
]}

Erben, Christoph M. ^{[3
]}

Turberfield, Andrew J. ^{[3
]}

Namba, Keiichi ^{[1
,2
]}

机构：

[1] Osaka Univ, Grad Sch Frontier Biosci, Osaka, Japan

[2] JST, ICORP, Dynam Nanomachine Project, Suita, Osaka 5650871, Japan

[3] Univ Oxford, Dept Phys, Clarendon Lab, Oxford OX1 3PU, England

来源：

NANO LETTERS | 2009年 / 9卷 / 07期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

SINGLE-PARTICLE RECONSTRUCTION; ELECTRON CRYOMICROSCOPY; CRYSTALS; RIBOSOME; COMPLEX; CAGE;

D O I：

10.1021/nl901265n

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Many DNA nanostructures have been produced and a wide range of potential applications have been proposed. However, confirmation of accurate 3D construction is particularly challenging. Here, we demonstrate that cryoEM may be exploited to obtain structural information at sufficient resolution to visualize the DNA helix and reveal the absolute stereochemistry of a 7 nm self-assembled DNA tetrahedron. Structural analysis at such high resolution by cryoEM image aim-lysis is unprecedented for any biological molecule of this size.

引用

收藏

页码：2747 / 2750

页数：4

相关论文

共 27 条

[1] MOLECULAR COMPUTATION OF SOLUTIONS TO COMBINATORIAL PROBLEMS [J].

ADLEMAN, LM .

SCIENCE, 1994, 266 (5187) :1021-1024

[2] Assembly and structural analysis of a covalently closed nano-scale DNA cage [J].

Andersen, Felicie F. ;

Knudsen, Bjarne ;

Oliveira, Cristiano Luis Pinto ;

Frohlich, Rikke F. ;

Kruger, Dinna ;

Bungert, Jorg ;

Agbandje-McKenna, Mavis ;

McKenna, Robert ;

Juul, Sissel ;

Veigaard, Christopher ;

Koch, Jorn ;

Rubinstein, John L. ;

Guldbrandtsen, Bernt ;

Hede, Marianne S. ;

Karlsson, Goran ;

Andersen, Anni H. ;

Pedersen, Jan Skov ;

Knudsen, Birgitta R. .

NUCLEIC ACIDS RESEARCH, 2008, 36 (04) :1113-1119

[3] DNA nanomachines [J].

Bath, Jonathan ;

Turberfield, Andrew J. .

NATURE NANOTECHNOLOGY, 2007, 2 (05) :275-284

[4] An autonomous molecular computer for logical control of gene expression [J].

Benenson, Y ;

Gil, B ;

Ben-Dor, U ;

Adar, R ;

Shapiro, E .

NATURE, 2004, 429 (6990) :423-429

[5] Single particle reconstruction of the human apo-transferrin-transferrin receptor complex [J].

Cheng, YF ;

Zak, O ;

Aisen, P ;

Harrison, SC ;

Walz, T .

JOURNAL OF STRUCTURAL BIOLOGY, 2005, 152 (03) :204-210

[6] Electron cryomicroscopy of biological machines at subnanometer resolution [J].

Chiu, W ;

Baker, ML ;

Jiang, W ;

Dougherty, M ;

Schmid, MF .

STRUCTURE, 2005, 13 (03) :363-372

[7] Single-molecule protein encapsulation in a rigid DNA cage [J].

Erben, Christoph M. ;

Goodman, Russell P. ;

Turberfield, Andrew J. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (44) :7414-7417

[8] Rapid chiral assembly of rigid DNA building blocks for molecular nanofabrication [J].

Goodman, RP ;

Schaap, IAT ;

Tardin, CF ;

Erben, CM ;

Berry, RM ;

Schmidt, CF ;

Turberfield, AJ .

SCIENCE, 2005, 310 (5754) :1661-1665

[9] Hierarchical self-assembly of DNA into symmetric supramolecular polyhedra [J].

He, Yu ;

Ye, Tao ;

Su, Min ;

Zhang, Chuan ;

Ribbe, Alexander E. ;

Jiang, Wen ;

Mao, Chengde .

NATURE, 2008, 452 (7184) :198-U41

[10] Exploration of parameters in cryo-EM leading to an improved density map of the E-coli ribosome [J].

LeBarron, Jamie ;

Grassucci, Robert A. ;

Shaikh, Tanvir R. ;

Baxter, William T. ;

Sengupta, Jayati ;

Frank, Joachim .

JOURNAL OF STRUCTURAL BIOLOGY, 2008, 164 (01) :24-32