Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture

被引：33

作者：

Abbah, Sunny A. ^{[1
,2
]}

Thomas, Dilip ^{[2
,3
]}

Browne, Shane ^{[2
]}

O'Brien, Timothy ^{[2
,3
]}

Pandit, Abhay ^{[2
]}

Zeugolis, Dimitrios I. ^{[1
,2
]}

机构：

[1] Natl Univ Ireland Galway, Regenerat Modular & Dev Engn Lab REMODEL, Biosci Res Bldg, Galway, Ireland

[2] Natl Univ Ireland Galway, Ctr Res Med Devices CURAM, Biosci Res Bldg, Galway, Ireland

[3] Natl Univ Ireland Galway, Regenerat Med Inst REMEDI, Biosci Res Bldg, Galway, Ireland

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

爱尔兰科学基金会;

关键词：

GROWTH-FACTOR-BETA; CELLS IN-VITRO; TGF-BETA; DIFFERENTIAL REGULATION; TENDON; COLLAGEN; DELIVERY; TISSUE; SCAFFOLDS; FIBROSIS;

D O I：

10.1038/srep20922

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-beta 1). In tendon tissue repair, increased activity of TGF-beta 1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by exerting a neutralising effect via downregulation of TGF-beta 1. Herein, we report that the delivery of DCN and IL-10 transgenes from a collagen hydrogel system supresses the constitutive expression of TGF-beta 1 and a range of pro-fibrotic extracellular matrix genes.

引用

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