Two-dimensional electrophoresis of plant proteins and standardization of gel patterns

被引：51

作者：

Tsugita, A ^{[1
]}

Kamo, M ^{[1
]}

Kawakami, T ^{[1
]}

Ohki, Y ^{[1
]}

机构：

[1] SCI UNIV TOKYO,FAC PHARMACEUT SCI,SHINJUKU KU,TOKYO 162,JAPAN

来源：

ELECTROPHORESIS | 1996年 / 17卷 / 05期

关键词：

two-dimensional polyacrylamide gel electrophoresis; Arabidopsis thaliana; rice; standardization by stripe type markers; deblocking with heptafluorobutyric acid vapor;

D O I：

10.1002/elps.1150170507

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins of two plants, Arabidopsis thaliana and rice (Oryza sativa) were subjected to two-dimensional electrophoresis analysis with two modifications: (i) comigration of external standard marker proteins with resultant horizontal and vertical stripes in the gel, and (ii) deblocking with a vapor of aqueous heptafluorobutyric acid for N-acetylserine. Approximately 5000 protein spots were separated from both the five tissues of Arabidopsis and the nine tissues of rice. Over one hundred spots were electroblotted for N-terminal sequencing. Among the newly sequenced proteins, 62 were from Arabidopsis and 51 from rice.

引用

页码：855 / 865

页数：11

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