High-speed separation of oleanolic acid and ursolic acid from the fruit of Ligustrum lucidum ait and crataegus by cyclodextrin-modified micellar electrokinetic chromatography

被引：7

作者：

Hu, YN ^{[1
]}

Wang, YM ^{[1
]}

Luo, G ^{[1
]}

Wei, W ^{[1
]}

机构：

[1] Tsing Hua Univ, Dept Chem, Beijing 100084, Peoples R China

来源：

ANALYTICAL LETTERS | 2000年 / 33卷 / 02期

基金：

中国国家自然科学基金;

关键词：

separation; micellar electrokinetic chromatography; oleanolic acid; ursolic acid;

D O I：

10.1080/00032710008543058

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) technique for determining oleanolic acid and ursolic acid in the fruit of Ligustrum lucidum Ait and Crataegus was developed. The two bio-active components were successfully separated within 4 minutes using a pH 8.5 sodium berate buffer containing 60mM SDS and 2mM gamma-CD. The correlation coefficients of the linear calibration graphs for the analyses exceeded 0.9980. The effects of pH, SDS, gamma-CD, sodium berate and temperature on separation were also investigated.

引用

页码：357 / 371

页数：15

共 11 条

[1]

DA Y, 1988, CHIN TRAD HERB DRUGS, V9, P562

[2] SEPARATION OF BIOACTIVE QUADRI-TERPENIC ACIDS FROM THE FRUIT OF LIGUSTRUM-LUCIDUM AIT BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY [J].

DU, QZ ;

XIONG, XP ;

ITO, Y .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1995, 18 (10) :1997-2004

[3] Determination of cinnamaldehyde, cinnamic acid, paeoniflorin, glycyrrhizin and [6]-gingerol in the traditional Chinese medicinal preparation Kuei-chih-tang by cyclodextrin-modified micellar electrokinetic chromatography [J].

Huang, HY ;

Kuo, KL ;

Hsieh, YZ .

JOURNAL OF CHROMATOGRAPHY A, 1997, 771 (1-2) :267-274

[4] DETERMINATION OF THE 6 MAJOR FLAVONOIDS IN SCUTELLARIAE RADIX BY MICELLAR ELECTROKINETIC CAPILLARY ELECTROPHORESIS [J].

LIU, YM ;

SHEU, SJ .

ANALYTICA CHIMICA ACTA, 1994, 288 (03) :221-226

[5]

LIU YM, 1993, J CHROMATOGR, V639, P323

[6] DETERMINATION OF EPHEDRINE ALKALOIDS BY CAPILLARY ELECTROPHORESIS [J].

LIU, YM ;

SHEU, SJ .

JOURNAL OF CHROMATOGRAPHY, 1992, 600 (02) :370-372

[7] DETERMINATION OF QUATERNARY ALKALOIDS FROM COPTIDIS RHIZOMA BY CAPILLARY ELECTROPHORESIS [J].

LIU, YM ;

SHEU, SJ .

JOURNAL OF CHROMATOGRAPHY, 1992, 623 (01) :196-199

[8]

LIU YM, 1993, J CHROMATOGR, V634, P329

[9]

LU SH, 1993, CHIN PHARM ANAL, V13, P291

[10]

Shi L. F., 1995, Acta Pharmaceutica Sinica, V30, P935

← 1 2 →