Agrobacterium-mediated transformation of cereals:: a promising approach crossing barriers

被引:132
作者
Shrawat, Ashok Kumar [1 ]
Loerz, Horst [1 ]
机构
[1] Univ Hamburg, Ctr Appl Plant Mol Biol AMP 2, D-22609 Hamburg, Germany
关键词
Agrobacterium; Agrobacterium-mediated transformation; cereal crops; cereal transformation; genetic transformation; regeneration;
D O I
10.1111/j.1467-7652.2006.00209.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cereal crops have been the primary targets for improvement by genetic transformation because of their worldwide importance for human consumption. For a long time, many of these important cereals were difficult to genetically engineer, mainly as a result of their inherent limitations associated with the resistance to Agrobacterium infection and their recalcitrance to in vitro regeneration. The delivery of foreign genes to rice plants via Agrobacterium tumefaciens has now become a routine technique. However, there are still serious handicaps with Agrobacterium-mediated transformation of other major cereals. In this paper, we review the pioneering efforts, existing problems and future prospects of Agrobacterium-mediated genetic transformation of major cereal crops, such as rice, maize, wheat, barley, sorghum and sugarcane.
引用
收藏
页码:575 / 603
页数:29
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