Increase of CD8+T-effector memory cells in peripheral blood of patients with relapsing-remitting multiple sclerosis compared to healthy controls

被引:35
作者
Haegele, K. F.
Stueckle, C. A.
Malin, J. -P.
Sindern, E.
机构
[1] Ruhr Univ Bochum, Dept Neurol, BG Kliniken Bergmannsheil, D-44789 Bochum, Germany
[2] Ruhr Univ Bochum, Marien Hosp Herne, Dept Radiol, D-44625 Herne, Germany
关键词
multiple sclerosis; T-cells; CCR7; CD45RA; effector-function; flow cytometry;
D O I
10.1016/j.jneuroim.2006.09.008
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
CCR7 and CD45RA expression on CD4+ and CD8+ T-cells in blood (PB) of 16 patients with multiple sclerosis (MS) and 16 healthy controls and cerebrospinal fluid (CSF) of 10 patients suffering from MS were analysed by flow cytometric measurements. T-cells were divided by their distinct homing potentials and effector-functions in three groups: naive T-cells (CCR7+, CD45PA+), central memory T-cells (T-CM) (CCR7+, CD45RA-) and effector memory T-cells (T-EM) (CCR7-, CD45RAJ There was a significant increase of CD8+ T-EM-cells in PB of MS patients compared to healthy controls, indicating systemic immune activation. Further we found a relative depletion of CD8+ T-EM-cells in CSF of MS patients compared to matching blood samples, suggesting that these cells represent the effector arm of the immune response and infiltrate the brain tissue at the sites of inflammation. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:168 / 174
页数:7
相关论文
共 18 条
[1]   CD8+ T cells from patients with acute multiple sclerosis display selective increase of adhesiveness in brain venules:: a critical role for P-selectin glycoprotein ligand-1 [J].
Battistini, L ;
Piccio, L ;
Rossi, B ;
Bach, S ;
Galgani, S ;
Gasperini, C ;
Ottoboni, L ;
Ciabini, D ;
Caramia, MD ;
Bernardi, G ;
Laudanna, C ;
Scarpini, E ;
McEver, RP ;
Butcher, EC ;
Borsellino, G ;
Constantin, G .
BLOOD, 2003, 101 (12) :4775-4782
[2]   Homing phenotypes of tumor-specific CD8 T cells are predetermined at the tumor site by crosspresenting APCs [J].
Calzascia, T ;
Masson, F ;
Di Berardino-Besson, W ;
Contassot, E ;
Wilmotte, R ;
Aurrand-Lions, M ;
Rüegg, C ;
Dietrich, PY ;
Walker, PR .
IMMUNITY, 2005, 22 (02) :175-184
[3]   CCR7 expression and memory T cell diversity in humans [J].
Campbell, JJ ;
Murphy, KE ;
Kunkel, EJ ;
Brightling, CE ;
Soler, D ;
Shen, ZM ;
Boisvert, J ;
Greenberg, HB ;
Vierra, MA ;
Goodman, SB ;
Genovese, MC ;
Wardlaw, AJ ;
Butcher, EC ;
Wu, LJ .
JOURNAL OF IMMUNOLOGY, 2001, 166 (02) :877-884
[4]   Autoreactive CD8+ T cells in multiple sclerosis:: a new target for therapy? [J].
Friese, MA ;
Fugger, L .
BRAIN, 2005, 128 :1747-1763
[5]   Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS [J].
Giunti, D ;
Borsellino, G ;
Benelli, R ;
Marchese, M ;
Capello, E ;
Valle, MT ;
Pedemonte, E ;
Noonan, D ;
Albini, A ;
Bernardi, G ;
Mancardi, GL ;
Battistini, L ;
Uccelli, A .
JOURNAL OF LEUKOCYTE BIOLOGY, 2003, 73 (05) :584-590
[6]  
GOLD R, 2004, INT SCI M WURZB JAN, V152, P1
[7]  
HAEGELE KF, 2004, CLIN LAB INT, V28, P18
[8]   Expression of CCR7 in multiple sclerosis:: Implications for CNS immunity [J].
Kivisäkk, P ;
Mahad, DJ ;
Callahan, MK ;
Sikora, K ;
Trebst, C ;
Tucky, B ;
Wujek, J ;
Ravid, R ;
Staugaitis, SM ;
Lassmann, H ;
Ransohoff, RM .
ANNALS OF NEUROLOGY, 2004, 55 (05) :627-638
[9]   Defining the clinical course of multiple sclerosis: Results of an international survey [J].
Lublin, FD ;
Reingold, SC .
NEUROLOGY, 1996, 46 (04) :907-911
[10]   Chemokines - Chemotactic cytokines that mediate inflammation [J].
Luster, AD .
NEW ENGLAND JOURNAL OF MEDICINE, 1998, 338 (07) :436-445