The crystal structure of oxylipin-conjugated barley LTP1 highlights the unique plasticity of the hydrophobic cavity of these plant lipid-binding proteins

被引：23

作者：

Bakan, B. ^{[1
]}

Hamberg, M. ^{[4
]}

Larue, V. ^{[2
,3
]}

Prange, T. ^{[2
,3
]}

Marion, D.

Lascombe, M. -B. ^{[2
,3
]}

机构：

[1] INRA, Unite Rech Biopolymeres, BIA, F-44316 Nantes 3, France

[2] Univ Paris 05, CNRS, UMR 8015, Lab Cristallog,Fac Pharm, F-75006 Paris, France

[3] Univ Paris 05, CNRS, UMR 8015, RMN Biol,Fac Pharm, F-75006 Paris, France

[4] Karolinska Inst, Dept Med Biochem & Biophys, Stockholm, Sweden

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 390卷 / 03期

关键词：

Lipid transfer protein; Oxylipin; Lipid binding; MACROMOLECULAR STRUCTURES; MAXIMUM-LIKELIHOOD; DEFENSE RESPONSES; TOBACCO; 9-LIPOXYGENASE; ARABIDOPSIS; INVOLVEMENT; REFINEMENT;

D O I：

10.1016/j.bbrc.2009.10.049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The barley lipid transfer protein (LTP1) adducted by an a-ketol, (9-hydroxy-10-oxo-12(Z)-octadecenoic acid) exhibits an unexpected high lipid transfer activity. The crystal structure of this oxylipin-adducted LTP1, (LTP1b) was determined at 1.8 angstrom resolution. The covalently bound oxylipin was partly exposed at the surface of the protein and partly buried within the hydrophobic cavity. The structure of the oxylipidated LTP1 emphasizes the unique plasticity of the hydrophobic cavity of these plant lipid-binding proteins when compared to the other members of the family. The plasticity of the hydrophobic cavity and increase of its surface hydrophobicity induced by the oxylipin account for the improvement of the lipid transfer activity of LTP1b. These observations open new perspectives to explore the different biological functions of LTPs, including their allergenic properties. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：780 / 785

页数：6

共 30 条

[1] Specific adduction of plant lipid transfer protein by an allene oxide generated by 9-lipoxygenase and allene oxide synthase [J].

Bakan, Benedicte ;

Hamberg, Mats ;

Perrocheau, Ludivine ;

Maume, Daniel ;

Rogniaux, Helene ;

Tranquet, Olivier ;

Rondeau, Corinne ;

Blein, Jean-Pierre ;

Ponchet, Michel ;

Marion, Didier .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (51) :38981-38988

[2] A lipid transfer protein binds to a receptor involved in the control of plant defence responses [J].

Buhot, N ;

Douliez, JP ;

Jacquemard, A ;

Marion, D ;

Tran, V ;

Maume, BF ;

Milat, ML ;

Ponchet, M ;

Mikès, V ;

Kader, JC ;

Blein, JP .

FEBS LETTERS, 2001, 509 (01) :27-30

[3] Modulation of the biological activity of a tobacco LTP1 by lipid complexation [J].

Buhot, N ;

Gomès, E ;

Milat, ML ;

Ponchet, M ;

Marion, D ;

Lequeu, J ;

Delrot, S ;

Coutos-Thévenot, P ;

Blein, JP .

MOLECULAR BIOLOGY OF THE CELL, 2004, 15 (11) :5047-5052

[4] The crystal structure of a wheat nonspecific lipid transfer protein (ns-LTP1) complexed with two molecules of phospholipid at 2.1 Å resolution [J].

Charvolin, D ;

Douliez, JP ;

Marion, D ;

Cohen-Addad, C ;

Pebay-Peyroula, E .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 264 (02) :562-568

[5]

Collaborative Computational Project Number 4, 1994, ACTA CRYSTALLOGR, V50, P760

[6] Binding of two mono-acylated lipid monomers by the barley lipid transfer protein, LTP1, as viewed by fluorescence, isothermal titration calorimetry and molecular modelling [J].

Douliez, JP ;

Jégou, S ;

Pato, C ;

Mollé, D ;

Tran, V ;

Marion, D .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (02) :384-388

[7] Identification of a new form of lipid transfer protein (LTP1) in wheat seeds [J].

Douliez, JP ;

Jégou, S ;

Pato, C ;

Larré, C ;

Mollé, D ;

Marion, D .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (04) :1805-1808

[8] Coot:: model-building tools for molecular graphics [J].

Emsley, P ;

Cowtan, K .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 :2126-2132

[9]

Heinemann B, 1996, PROTEIN SCI, V5, P13

[10] Lipid-transfer proteins in plants [J].

Kader, JC .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1996, 47 :627-654

← 1 2 3 →