Arabidopsis thaliana histone deacetylase 1 (AtHD1) is localized in euchromatic regions and demonstrates histone deacetylase activity in vitro

被引：34

作者：

Fong, Paulus M. ^{[1
]}

Tian, Lu ^{[1
]}

Chen, Z. Jeffrey ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA

来源：

CELL RESEARCH | 2006年 / 16卷 / 05期

关键词：

Arabidopsis; histone deacetylase; RPD3; subcellular localization; gene silencing; chromatin;

D O I：

10.1038/sj.cr.7310059

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Arabidopsis thaliana histone deacetylase 1 (AtHD1 or AtHDA19), a homolog of yeast RPD3, is a global regulator of many physiological and developmental processes in plants. In spite of the genetic evidence for a role of AtHD1 in plant gene regulation and development, the biochemical and cellular properties of AtHD1 are poorly understood. Here we report cellular localization patterns of AtHD1 in vivo and histone deacetylase activity in vitro. The transient and stable expression of a green fluorescent protein (GFP)-tagged AtHD1 in onion cells and in roots, seeds and leaves of the transgenic Arabidopsis, respectively, revealed that AtHD1 is localized in the nucleus presumably in the euchromatic regions and excluded from the nucleolus. The localization patterns of AtHD1 are different from those of AtHD2 and AtHDA6 that are involved in nucleolus formation and silencing of transgenes and repeated DNA elements, respectively. In addition, a histone deacetylase activity assay showed that the recombinant AtHD1 produced in bacteria demonstrated a specific histone deacetylase activity in vitro. The data suggest that AtHD1 is a nuclear protein and possesses histone deacetylase activities responsible for global transcriptional regulation important to plant growth and development.

引用

页码：479 / 488

页数：10

共 62 条

[31] The SUMO E3 ligase RanBP2 promotes modification of the HDAC4 deacetylase [J].

Kirsh, O ;

Seeler, JS ;

Pichler, A ;

Gast, A ;

Müller, S ;

Miska, E ;

Mathieu, M ;

Harel-Bellan, A ;

Kouzarides, T ;

Melchior, F ;

Dejean, A .

EMBO JOURNAL, 2002, 21 (11) :2682-2691

[32] High-throughput protein localization in Arabidopsis using Agrobacterium-mediated transient expression of GFP-ORF fusions [J].

Koroleva, OA ;

Tomlinson, ML ;

Leader, D ;

Shaw, P ;

Doonan, JH .

PLANT JOURNAL, 2005, 41 (01) :162-174

[33]

Kruhlak MJ, 2001, J BIOL CHEM, V276, P38307

[34] A concerted DNA methylation/histone methylation switch regulates rRNA gene dosage control and nucleolar dominance [J].

Lawrence, RJ ;

Earley, K ;

Pontes, O ;

Silva, M ;

Chen, ZJ ;

Neves, N ;

Viegas, W ;

Pikaard, CS .

MOLECULAR CELL, 2004, 13 (04) :599-609

[35] Negative regulation of histone deacetylase 8 activity by cyclic AMP-dependent protein kinase A [J].

Lee, H ;

Rezai-Zadeh, N ;

Seto, E .

MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (02) :765-773

[36] Dynamic distribution of Ser-10 phosphorylated histone H3 in cytoplasm of MCF-7 and CHO cells during mitosis [J].

Li, DW ;

Yang, Q ;

Chen, JT ;

Zhou, H ;

Liu, RM ;

Huang, XT .

CELL RESEARCH, 2005, 15 (02) :120-126

[37] Distinct mechanisms determine transposon inheritance and methylation via small interfering RNA and histone modification [J].

Lippman, Z ;

May, B ;

Yordan, C ;

Singer, T ;

Martienssen, R .

PLOS BIOLOGY, 2003, 1 (03) :420-428

[38] A plant dialect of the histone language [J].

Loidl, P .

TRENDS IN PLANT SCIENCE, 2004, 9 (02) :84-90

[39] Identification of maize histone deacetylase HD2 as an acidic nucleolar phosphoprotein [J].

Lusser, A ;

Brosch, G ;

Loidl, A ;

Haas, H ;

Loidl, P .

SCIENCE, 1997, 277 (5322) :88-91

[40] THE CENTROMERE AND PROMOTER FACTOR-I, CPF1, OF SACCHAROMYCES-CEREVISIAE MODULATES GENE ACTIVITY THROUGH A FAMILY OF FACTORS INCLUDING SPT21, RPD1 (SIN3), RPD3 AND CCR4 [J].

MCKENZIE, EA ;

KENT, NA ;

DOWELL, SJ ;

MORENO, F ;

BIRD, LE ;

MELLOR, J .

MOLECULAR & GENERAL GENETICS, 1993, 240 (03) :374-386

← 1 2 3 4 5 6 7 →