Distinct Targets of the Eco1 Acetyltransferase Modulate Cohesion in S Phase and in Response to DNA Damage

被引：113

作者：

Heidinger-Pauli, Jill M. ^{[1
,2
,3
]}

Uenal, Elcin ^{[1
,2
,3
]}

Koshland, Douglas ^{[1
,2
]}

机构：

[1] Carnegie Inst Washington, Howard Hughes Med Inst, Baltimore, MD 21218 USA

[2] Carnegie Inst Washington, Dept Embryol, Baltimore, MD 21218 USA

[3] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

来源：

MOLECULAR CELL | 2009年 / 34卷 / 03期

关键词：

SISTER-CHROMATID COHESION; DOUBLE-STRAND BREAKS; SACCHAROMYCES-CEREVISIAE; DROSOPHILA-MELANOGASTER; ESTABLISHMENT; REPLICATION; ACETYLATION; PROTEIN; YEAST; HISTONE;

D O I：

10.1016/j.molcel.2009.04.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromosome segregation and the repair of DNA double-strand breaks (DSBs) require cohesin, the protein complex that mediates sister chromatid cohesion. Cohesion requires both a chromatin binding step and a subsequent tethering step called cohesion generation. Here we provide insight into how cohesion generation is restricted to S phase but can be activated in G2/M by a DSB in budding yeast. We show that Wpl1p inhibits cohesion in G2/M. A DSB counteracts Wpl1p and stimulates cohesion generation by first inducing the phosphorylation of the Mcd1p subunit of cohesin. This phosphorylation activates Eco1p-dependent acetylation of Mcd1p, which in turn antagonizes Wpl1p. Previous studies show that Eco1p antagonizes Wpl1p in S phase by acetylating the Smc3p subunit of cohesin. We show that Mcd1p and Smc3p acetylation antagonize Wpl1p only in their proper context. Thus, Eco1p antagonizes Wpl1p in distinct ways to modulate cohesion generation during the cell cycle and after DNA damage.

引用

页码：311 / 321

页数：11

共 32 条

[31] Two putative acetyltransferases, San and deco, are required for establishing sister chromatid cohesion in drosophila [J].