Anti-inflammatory and Antiapoptotic Effect of Interleukine-18 Binding Protein on the Spinal Cord Ischemia-Reperfusion Injury

被引:27
作者
Karavelioglu, Ergun [1 ]
Gonul, Yucel [2 ]
Kokulu, Serdar [3 ]
Hazman, Omer [4 ]
Bozkurt, Fatih [5 ]
Kocak, Ahmet [6 ]
Eser, Olcay [7 ]
机构
[1] Afyon Kocatepe Univ, Fac Med, Dept Neurosurg, Afyon, Turkey
[2] Afyon Kocatepe Univ, Fac Med, Dept Anat, Afyon, Turkey
[3] Afyon Kocatepe Univ, Fac Med, Dept Anesthesia & Reanimat, Afyon, Turkey
[4] Afyon Kocatepe Univ, Fac Sci & Arts, Dept Chem, Afyon, Turkey
[5] Afyon Kocatepe Univ, Dept Pathol, Fac Vet, Afyon, Turkey
[6] Dumlupinar Univ, Dept Histol, Fac Med, Kutahya, Turkey
[7] Balikesir Univ, Dept Neurosurg, Fac Med, Balikesir, Turkey
关键词
interleukin-18 binding protein; spinal cord; ischemia-reperfusion; cytokines; caspase; 3; TUMOR-NECROSIS-FACTOR; PRO-INFLAMMATORY CYTOKINE; INDUCED LIVER-INJURY; ISCHEMIA/REPERFUSION INJURY; PROINFLAMMATORY CYTOKINES; MESSENGER-RNA; TNF-ALPHA; BRAIN; IL-18; TH1;
D O I
10.1007/s10753-014-9811-7
中图分类号
Q2 [细胞生物学];
学科分类号
071013 [干细胞生物学];
摘要
We investigated the anti-inflammatory and antiapoptotic effects of interleukin-18 binding protein (IL-18BP) on spinal cord ischemia/reperfusion (I/R) injury in rats. Twenty-one adult male rats were divided into three groups: sham, I/R, and I/R+IL-18BP. Proinflammatory cytokines were determined in rat blood samples by using ELISA, while apoptosis was immunohistochemically evaluated in spinal cord tissue using caspase 3. Both IL-18 and TNF-alpha were significantly decreased in the IL-18BP group compared to that in the sham group. The highest caspase 3 levels were observed in the I/R group, while the lowest levels were found in the sham group. The mean Tarlov score of the I/R group was significantly lower than that of the sham group. However, the mean Tarlov score of the IL-18BP group was significantly higher than that of the I/R group. The results of the current study demonstrate that IL-18BP plays both anti-inflammatory and antiapoptotic roles in spinal cord I/R injury.
引用
收藏
页码:917 / 923
页数:7
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