Anti-inflammatory and Antiapoptotic Effect of Interleukine-18 Binding Protein on the Spinal Cord Ischemia-Reperfusion Injury

被引：27

作者：

Karavelioglu, Ergun ^{[1
]}

Gonul, Yucel ^{[2
]}

Kokulu, Serdar ^{[3
]}

Hazman, Omer ^{[4
]}

Bozkurt, Fatih ^{[5
]}

Kocak, Ahmet ^{[6
]}

Eser, Olcay ^{[7
]}

机构：

[1] Afyon Kocatepe Univ, Fac Med, Dept Neurosurg, Afyon, Turkey

[2] Afyon Kocatepe Univ, Fac Med, Dept Anat, Afyon, Turkey

[3] Afyon Kocatepe Univ, Fac Med, Dept Anesthesia & Reanimat, Afyon, Turkey

[4] Afyon Kocatepe Univ, Fac Sci & Arts, Dept Chem, Afyon, Turkey

[5] Afyon Kocatepe Univ, Dept Pathol, Fac Vet, Afyon, Turkey

[6] Dumlupinar Univ, Dept Histol, Fac Med, Kutahya, Turkey

[7] Balikesir Univ, Dept Neurosurg, Fac Med, Balikesir, Turkey

来源：

INFLAMMATION | 2014年 / 37卷 / 03期

关键词：

interleukin-18 binding protein; spinal cord; ischemia-reperfusion; cytokines; caspase; 3; TUMOR-NECROSIS-FACTOR; PRO-INFLAMMATORY CYTOKINE; INDUCED LIVER-INJURY; ISCHEMIA/REPERFUSION INJURY; PROINFLAMMATORY CYTOKINES; MESSENGER-RNA; TNF-ALPHA; BRAIN; IL-18; TH1;

D O I：

10.1007/s10753-014-9811-7

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

We investigated the anti-inflammatory and antiapoptotic effects of interleukin-18 binding protein (IL-18BP) on spinal cord ischemia/reperfusion (I/R) injury in rats. Twenty-one adult male rats were divided into three groups: sham, I/R, and I/R+IL-18BP. Proinflammatory cytokines were determined in rat blood samples by using ELISA, while apoptosis was immunohistochemically evaluated in spinal cord tissue using caspase 3. Both IL-18 and TNF-alpha were significantly decreased in the IL-18BP group compared to that in the sham group. The highest caspase 3 levels were observed in the I/R group, while the lowest levels were found in the sham group. The mean Tarlov score of the I/R group was significantly lower than that of the sham group. However, the mean Tarlov score of the IL-18BP group was significantly higher than that of the I/R group. The results of the current study demonstrate that IL-18BP plays both anti-inflammatory and antiapoptotic roles in spinal cord I/R injury.

引用

页码：917 / 923

页数：7

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