Xyloglucan from soybean (Glycine max) meal is composed of XXXG-type building units

被引：24

作者：

Huisman, MMH ^{[1
]}

Weel, KGC ^{[1
]}

Schols, HA ^{[1
]}

Voragen, AGJ ^{[1
]}

机构：

[1] Agr Univ Wageningen, Dept Food Sci, NL-6703 HD Wageningen, Netherlands

来源：

CARBOHYDRATE POLYMERS | 2000年 / 42卷 / 02期

关键词：

xyloglucan; soybean meal; XXXG-type building unit;

D O I：

10.1016/S0144-8617(99)00154-X

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Soybean cell wall material was depectinated by extraction with a hot chelating agent and a cold dilute alkali. The hemicelluloses were solubilised from the residue with 1 and 4 M KOH solutions, resulting in 1 M Alkali Soluble Solids (1 MASS) and 4 M Alkali Soluble Solids (4 MASS) fractions. The polysaccharides extracted with 1 M KOH were fractionated by ion-exchange chromatography, yielding a neutral and a pectic population. The sugar composition of the neutral population indicated the presence of xyloglucans and possibly xylans. Enzymatic degradation with endo-xylanases and endo-glucanases showed the presence of xyloglucans only. Analysis of the digest formed after incubation of the neutral population with endo-glucanase V using both HPAEC and MALDI-TOF MS showed the formation of the characteristic poly-XXXG xyloglucan oligomers (XXG, XXXG, XXFG, XLXG, and XLFG). (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：185 / 191

页数：7

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