Prospective evaluation of the GenoType Assay for routine identification of mycobacteria

被引:35
作者
Sarkola, A
Mäkinen, J
Marjamäki, M
Marttila, HJ
Viljanen, MK
Soini, H
机构
[1] Natl Publ Hlth Inst, Mycobacterial Reference Lab, Turku 20520, Finland
[2] Univ Turku, Dept Med, FIN-20520 Turku, Finland
[3] Univ Turku, Dept Med Microbiol, FIN-20520 Turku, Finland
关键词
D O I
10.1007/s10096-004-1168-7
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
In order to evaluate the proficiency of the GenoType Mycobacteria strip hybridization assay (Hain Lifescience, Nehren, Germany) for the routine identification of mycobacteria, the assay was used to identify 178 clinical isolates during a 6-month prospective study. The GenoType results were compared to the identification results obtained with AccuProbe (GenProbe, San Diego, CA, USA) or 16S rDNA sequencing, and an overall agreement of 89.3% between GenoType and the two reference methods was reached. The GenoType assay is, thus, a rapid and reliable method for the identification of clinically important mycobacteria, and it is well suited for use in a routine laboratory.
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收藏
页码:642 / 645
页数:4
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