Expression of cereolysine AB genes in Bacillus anthracis vaccine strain ensures protection against experimental hemolytic anthrax infection

被引:76
作者
Pomerantsev, AP
Staritsin, NA
Mockov, YV
Marinin, LI
机构
[1] Stt. Res. Ctr. for Appl. Microbiol., Obolensk
关键词
Bacillus anthracis; modulation of immunopathogenicity; Bacillus cereus;
D O I
10.1016/S0264-410X(97)00132-1
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The cereolysin AB genes from Bacillus cereus VKM-B164 have been expressed in Bacillus anthracis strains: virulent H-7 (PXO1, PXO2), vaccine STI-1 (PXO1), 221 (without its own plasmids). Expression was achieved by cloning the genes in a high copy number plasmid pE194. This construct was integrated with host genomes in amplified form. Gold hamsters were vaccinated with parental and recombinant B. anthracis STI-1 and 221 strains and challenged with virulent ones subcutaneously. Gold hamsters vaccinated with 221 strains and challenged with virulent ones subcutaneously. Gold hamsters vaccinated with 221 strains showed absence of protection. STI-1 immunisation protected against the H-7 strain, but did not protect against the recombinant strain. STI-1 recombinant strain protected gold hamsters against the H-7 as well as the recombinant H-7 strains. The results describe the modulation of immunopathogenic properties of B. anthracis due to expression of cereolysin AB genes. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码:1846 / 1850
页数:5
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