Chemically induced hairpin formation in DNA monolayers

被引：53

作者：

Smith, EA

Kyo, M

Kumasawa, H

Nakatani, K

Saito, I

Corn, RM

机构：

[1] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA

[2] Kyoto Univ, Fac Engn, Dept Synthet Chem & Biol Chem, Kyoto 6068501, Japan

[3] Japan Sci & Technol Corp, PREST, Kyoto 6068501, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 24期

关键词：

D O I：

10.1021/ja026356n

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A naphthyridine dimer that binds specifically to G-G mismatches has been used to induce hairpin formation in oligonucleotides immobilized onto chemically modified gold surfaces. Surface plasmon resonance (SPR) imaging measurements of DNA microarrays were used to demonstrate that binding of the naphthyridine dimer to G-G mismatches within the stem portion of an immobilized 42-mer oligonucleotide could be used to induce hairpin formation that prevented hybridization of DNA complementary to the loop sequence. In addition, the selectivity of the naphthyridine dimer for G-G mismatches was verified through SPR imaging measurements of the hybridization adsorption of an 11-mer oligonucleotide to a four-component DNA array of zero- and single-base mismatch sequences. Copyright © 2002 American Chemical Society.

引用

页码：6810 / 6811

页数：2

共 8 条

[1] A multistep chemical modification procedure to create DNA arrays on gold surfaces for the study of protein-DNA interactions with surface plasmon resonance imaging [J].