Antiplatelet effect of green tea catechins: a possible mechanism through arachidonic acid pathway

We have previously reported that green tea catechins (GTC) showed an antithrombotic activity, which might be due to antiplatelet effect rather than anticoagulation. The present study was performed to investigate the effect of GTC on the arachidonic acid (AA) metabolism in order to elucidate a possible antiplatelet mechanism. GTC inhibited the collagen-, AA- and U46619-induced rabbit platelet aggregation in vitro in a concentration-dependent manner, with IC50 values of 61.0 +/- 2.5, 105.0 +/- 4.9 and 67.0 +/- 3.2 mug/ml, respectively. Moreover, GTC administered orally into rats inhibited the AA-induced platelet aggregation ex vivo by 46.9 +/- 6.1% and 95.4 +/- 2.2% at the doses of 25 and 50 mg/kg, respectively. [H-3]AA liberation induced by collagen in [H-3]AA incorporated rabbit platelets was significantly suppressed by GTC compared to the control. GTC also significantly inhibited the thromboxane A(2) (TXA(2)) and prostaglandin D-2 (PGD(2)) generations induced by addition of AA in intact rabbit platelets. GTC significantly inhibited TXA(2) synthase activity in a concentration-dependent manner. Moreover, adenosine triphosphate (ATP) release from dense granule was inhibited by GTC in washed platelets. These results suggest that the antiplatelet activity of GTC may be due to the inhibition of TXA(2) formation through the inhibition of AA liberation and TXA(2) synthase. (C) 2004 Elsevier Ltd. All rights reserved.