Embryonic stem cell-specific microRNAs promote induced pluripotency

被引：520

作者：

Judson, Robert L.

Babiarz, Joshua E.

Venere, Monica

Blelloch, Robert ^{[1
]}

机构：

[1] Univ Calif San Francisco, Eli & Edythe Broad, Ctr Regenerat Med & Stem Cell Res, Ctr Reprod Sci, San Francisco, CA 94143 USA

来源：

NATURE BIOTECHNOLOGY | 2009年 / 27卷 / 05期

关键词：

DNA METHYLATION; MOUSE; GENERATION; CLUSTER;

D O I：

10.1038/nbt.1535

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This report demonstrates that introduction of microRNAs (miRNAs) specific to embryonic stem cells enhances the production of mouse induced pluripotent stem (iPS) cells. The miRNAs miR-291-3p, miR-294 and miR-295 increase the efficiency of reprogramming by Oct4, Sox2 and Klf4, but not by these factors plus cMyc. cMyc binds the promoter of the miRNAs, suggesting that they are downstream effectors of cMyc during reprogramming. However, unlike cMyc, the miRNAs induce a homogeneous population of iPS cell colonies.

引用

页码：459 / 461

页数：3

共 17 条

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