Genotoxic effects of ochratoxin A in human-derived hepatoma (HepG2) cells

被引：72

作者：

Ehrlich, V

Darroudi, F

Uhl, M

Steinkellner, H

Gann, M

Majer, BJ

Eisenbauer, M

Knasmüller, S

机构：

[1] Univ Vienna, Inst Canc Res, A-1090 Vienna, Austria

[2] Leiden State Univ, Med Ctr, Dept Radiat Genet & Chem Mutagenesis, NL-2333 AL Leiden, Netherlands

[3] Inst Bldg Biol & Ecol, A-1090 Vienna, Austria

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2002年 / 40卷 / 08期

关键词：

ochratoxin A; HepG2; micronouclei; comet assay; Ames test; HepG2-S9;

D O I：

10.1016/S0278-6915(02)00045-5

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Ochratoxin A (OTA) is a widespread mycotoxin that occurs in many commodities from grains to coffee beans all over the world. Evidence is accumulating that OTA may cause cancer in humans. The compound was tested in micronucleus (MN) and single-cell gel electrophoresis (SCGE) assays in human-derived hepatoma (HepG2) cells and caused pronounced dose-dependent effects at exposure concentrations of 5 mug/ml and greater. On the contrary, no induction of His(+) revertants was found in Salmonella microsome assays with strains TA98 and TA100 with HepG2-derived enzyme (S9) mix in liquid incubation assays under identical exposure concentrations. Taken together, our results indicate that OTA is clastogenic in the human-derived cells. These findings support the assumption that this mycotoxin may cause genotoxic effects in hepatic tissue of humans. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1085 / 1090

页数：6

共 42 条

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