Seeing is believing: new methods for in situ single-cell transcriptomics

被引:11
作者
Avital, Gal [1 ]
Hashimshony, Tamar [1 ]
Yanai, Itai [1 ]
机构
[1] Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel
关键词
RNA-SEQ; EXPRESSION; TISSUE;
D O I
10.1186/gb4169
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
New methods employ RNA-seq to study single cells within complex tissues by in situ sequencing or mRNA capture from single photoactivated cells.
引用
收藏
页数:4
相关论文
共 10 条
[1]   ANALYSIS OF GENE-EXPRESSION IN SINGLE LIVE NEURONS [J].
EBERWINE, J ;
YEH, H ;
MIYASHIRO, K ;
CAO, YX ;
NAIR, S ;
FINNELL, R ;
ZETTEL, M ;
COLEMAN, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (07) :3010-3014
[2]   Transcriptome sequencing of single cells with Smart-Seq [J].
Goetz, Jillian J. ;
Trimarchi, Jeffrey M. .
NATURE BIOTECHNOLOGY, 2012, 30 (08) :763-765
[3]   CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification [J].
Hashimshony, Tamar ;
Wagner, Florian ;
Sher, Noa ;
Yanai, Itai .
CELL REPORTS, 2012, 2 (03) :666-673
[4]   Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq [J].
Islam, Saiful ;
Kjallquist, Una ;
Moliner, Annalena ;
Zajac, Pawel ;
Fan, Jian-Bing ;
Lonnerberg, Peter ;
Linnarsson, Sten .
GENOME RESEARCH, 2011, 21 (07) :1160-1167
[5]   In situ sequencing for RNA analysis in preserved tissue and cells [J].
Ke, Rongqin ;
Mignardi, Marco ;
Pacureanu, Alexandra ;
Svedlund, Jessica ;
Botling, Johan ;
Wahlby, Carolina ;
Nilsson, Mats .
NATURE METHODS, 2013, 10 (09) :857-+
[6]  
Kivioja T, 2012, NAT METHODS, V9, P72, DOI [10.1038/NMETH.1778, 10.1038/nmeth.1778]
[7]   Highly Multiplexed Subcellular RNA Sequencing in Situ [J].
Lee, Je Hyuk ;
Daugharthy, Evan R. ;
Scheiman, Jonathan ;
Kalhor, Reza ;
Yang, Joyce L. ;
Ferrante, Thomas C. ;
Terry, Richard ;
Jeanty, Sauveur S. F. ;
Li, Chao ;
Amamoto, Ryoji ;
Peters, Derek T. ;
Turczyk, Brian M. ;
Marblestone, Adam H. ;
Inverso, Samuel A. ;
Bernard, Amy ;
Mali, Prashant ;
Rios, Xavier ;
Aach, John ;
Church, George M. .
SCIENCE, 2014, 343 (6177) :1360-1363
[8]  
Lovatt D, 2014, NAT METHODS, V11, P190, DOI [10.1038/NMETH.2804, 10.1038/nmeth.2804]
[9]   Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells [J].
Shalek, Alex K. ;
Satija, Rahul ;
Adiconis, Xian ;
Gertner, Rona S. ;
Gaublomme, Jellert T. ;
Raychowdhury, Raktima ;
Schwartz, Schraga ;
Yosef, Nir ;
Malboeuf, Christine ;
Lu, Diana ;
Trombetta, John J. ;
Gennert, Dave ;
Gnirke, Andreas ;
Goren, Alon ;
Hacohen, Nir ;
Levin, Joshua Z. ;
Park, Hongkun ;
Regev, Aviv .
NATURE, 2013, 498 (7453) :236-240
[10]  
Tang FC, 2011, NAT METHODS, V8, pS6, DOI [10.1038/NMETH.1557, 10.1038/nmeth.1557]