Isolate specific detection of mycorrhizal fungi using genome specific primer pairs

被引：19

作者：

Abbas, JD

Hetrick, BAD

Jurgenson, JE

机构：

[1] Department of Biology, University of Northern Iowa, Cedar Falls

来源：

MYCOLOGIA | 1996年 / 88卷 / 06期

关键词：

Glomus mosseae; Gigaspora margarita; DNA sequencing; Mycorrhiza; PCR; RAPD; Zygomycetes;

D O I：

10.2307/3761056

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Random amplification of polymorphic DNA analysis was used to generate DNA fragments that are unique to isolates of two arbuscular mycorrhizal fungi, Glomus mosseae and Gigaspora margarita. Sequence analysis of these fragments allowed generation of primer pairs, and subsequent specific identification of these genomes even in the presence of competing genomic DNA's. This approach can be used to specifically detect the presence of these fungi in mixtures of spores or infected roots.

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收藏

页码：939 / 946

页数：8

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