Effect of hypoxia on the immune response of giant freshwater prawn Macrobrachium rosenbergii and its susceptibility to pathogen Enterococcus

被引:105
作者
Cheng, W
Liu, CH
Hsu, JP
Chen, JC [1 ]
机构
[1] Natl Taiwan Ocean Univ, Dept Aquaculture, Chilung 202, Taiwan
[2] Natl Pingtung Univ Sci & Technol, Dept Aquaculture, Pingtung 912, Taiwan
[3] Natl Pingtung Univ Sci & Technol, Inst Trop Agr, Pingtung 912, Taiwan
[4] Pingtung Hsien Livestock Dis Control Ctr, Pingtung 900, Taiwan
关键词
Macrobrachium rosenbergii; Enterococcus; dissolved oxygen; challenge; virulence; haemocyte count; phenoloxidase activity; respiratory burst; phagocytosis; clearance efficiency;
D O I
10.1006/fsim.2001.0411
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Giant freshwater prawns Macrobrachium rosenbergii (14-19 g) were challenged with Enterococcus (3 x 10(5) cfu prawn(-1)) previously incubated in TSB medium for 24 h, then placed in water having concentrations of dissolved oxygen (DO) at 7.75, 4.75, 2.75 and 1.75 mg l(-1). Onset of mortality occurred after 6 h exposure to 1.75 mg l(-1) DO, and after 12 h exposure to 2.75 mg l(-1) DO. Cumulative mortality of prawns at 1.75 mg l(-1) DO was significantly higher than that at 4.75 and 2.75 mg l(-1) DO, and cumulative mortality of prawns at 4-75 and 2-75 mg l(-1) DO was significantly higher than that at 7-75 mg l(-1) DO after 96 h. The prawns (20-30 g) which had been placed in water for 0 to 120 h at 7.75, 4.75, 2.75 and 1.75 mg l(-1) DO were examined for the THC (total haemocyte counts), DHC (differential haemocyte counts), phenoloxidase activity, respiratory burst, percentage phagocytosis and clearance efficiency, No significant difference in semi-granular cells and granular cells of prawns was observed among four treatments. The prawns following 120 h exposure to 2-75 mg l(-1) DO decreased significantly the hyaline cells and THC by 39% and 36%, respectively. Phenoloxidase activity and respiratory burst decreased significantly by 33% and 11% when the prawns were exposed to 2-75 mg l(-1) DO after 24 h, respectively. Percentage phagocytosis and clearance efficiency to Enterococcus decreased significantly by 44% and 54% for the prawns following 12h exposure to 2.75 mg l(-1) DO, respectively. It is concluded that DO as low as 2.75 mg l(-1) and 4.75 mg l(-1) causes depression in immune system of M. rosenbergii, and increases its susceptibility to Enterococcus infection. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:351 / 365
页数:15
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