Chemoselective 15N Tag for Sensitive and High-Resolution Nuclear Magnetic Resonance Profiling of the Carboxyl-Containing Metabolome

被引：48

作者：

Ye, Tao ^{[1
]}

Mo, Huaping ^{[2
,3
]}

Shanaiah, Narasimhamurthy ^{[1
]}

Gowda, G. A. Nagana ^{[1
]}

Zhang, Shucha ^{[1
]}

Raftery, Daniel ^{[1
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[2] Purdue Univ, Purdue Interdepartmental NMR Facil, W Lafayette, IN 47907 USA

[3] Purdue Univ, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 12期

基金：

美国国家卫生研究院;

关键词：

2-DIMENSIONAL GAS-CHROMATOGRAPHY; FLIGHT MASS-SPECTROMETRY; NMR-SPECTROSCOPY; CONDENSING AGENT; ACID METABOLITES; SYSTEMS BIOLOGY; ORGANIC-ACIDS; BLOOD-PLASMA; TOCSY; QUANTIFICATION;

D O I：

10.1021/ac900539y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Metabolic profiling has received increasing recognition as an indispensable complement to genomics and proteomics for probing biological systems and for clinical applications. H-1 nuclear magnetic resonance (NMR) is widely used in the field but is challenged by spectral complexity and overlap. Improved and simple methods that quantitatively profile a large number of metabolites are sought to make further progress. Here, we demonstrate a simple isotope tagging strategy, in which metabolites with carboxyl groups are chemically tagged with N-15-ethanolamine and detected using a 2D heteronuclear correlation NMR experiment. This method is capable of detecting over 100 metabolites at concentrations as low as a few micromolar in biological samples, both quantitatively and reproducibly. Carboxyl-containing compounds are found in almost all metabolic pathways, and thus this new approach should find a variety of applications.

引用

页码：4882 / 4888

页数：7

共 38 条

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