Determination of the metabolites of nitrofuran antibiotics in meat by liquid chromatography-mass spectrometry

被引：16

作者：

Hormazábal, V ^{[1
]}

Asp, TN ^{[1
]}

机构：

[1] Norwegian Sch Vet Sci, Div Food Safety, N-0033 Oslo, Norway

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2004年 / 27卷 / 17期

关键词：

nitrofuran antibiotics; meat; liquid chromatography-mass spectrometry;

D O I：

10.1081/JLC-200029335

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatographic-mass spectrometry (LC-MS) method for the simultaneous determination of metabolites from furaltadone, furazolidone, nitrofurantoin, and nitrofurazone in meat is described. Samples were Extracted with trichloroacetic acid (TCA) and further clean-up was achieved with solid phase extraction (SPE) after derivatisation with 2-nitrobenzaldehyde (NBA). The derivatised matabolites were eluted with acetonitrile-water and extracted with chloroform. The organic layer was evaporated to dryness and the residue was dissolved in water. The limits of detection varied from 0.2 to 0.5 ng/g.

引用

页码：2759 / 2770

页数：12

共 15 条

[1] Isolation of bound residues of nitrofuran drugs from tissue by solid-phase extraction with determination by liquid chromatography with UV and tandem mass spectrometric detection [J].

Conneely, A ;

Nugent, A ;

O'Keeffe, M ;

Mulder, PPJ ;

van Rhijn, JA ;

Kovacsics, L ;

Fodor, A ;

McCracken, RJ ;

Kennedy, DG .

ANALYTICA CHIMICA ACTA, 2003, 483 (1-2) :91-98

[2]

European Commission, 1990, J EUR COMMUN, VL224, P55

[3] DEPLETION AND BIOAVAILABILITY OF [C-14] FURAZOLIDONE RESIDUES IN SWINE TISSUES [J].

GOTTSCHALL, DW ;

WANG, R .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1995, 43 (09) :2520-2525

[4] THE USE OF PIG HEPATOCYTES TO STUDY THE NATURE OF PROTEIN-BOUND METABOLITES OF FURAZOLIDONE - A NEW ANALYTICAL METHOD FOR THEIR DETECTION [J].

HOOGENBOOM, LAP ;

VANKAMMEN, M ;

BERGHMANS, MCJ ;

KOEMAN, JH ;

KUIPER, HA .

FOOD AND CHEMICAL TOXICOLOGY, 1991, 29 (05) :321-328

[5]

HOOGENBOOM LAP, 1993, P EUR 2 C, P376

[6] Analysis of protein-bound metabolites of furazolidone and furaltadone in pig liver by high-performance liquid chromatography and liquid chromatography mass spectrometry [J].

Horne, E ;

Cadogan, A ;

OKeeffe, M ;

Hoogenboom, LAP .

ANALYST, 1996, 121 (10) :1463-1468

[7] Determination of the metabolites of nitrofuran antibiotics in animal tissue by high-performance liquid chromatography-tandem mass spectrometry [J].

Leitner, A ;

Zöllner, P ;

Lindner, W .

JOURNAL OF CHROMATOGRAPHY A, 2001, 939 (1-2) :49-58

[8] Determination of the furazolidone metabolite, 3-amino-2-oxazolidinone, in porcine tissues using liquid chromatography-thermospray mass spectrometry and the occurrence of residues in pigs produced in Northern Ireland [J].

McCracken, RJ ;

Kennedy, DG .

JOURNAL OF CHROMATOGRAPHY B, 1997, 691 (01) :87-94

[9] DETERMINATION OF FURAZOLIDONE IN PORCINE TISSUE USING THERMOSPRAY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY AND A STUDY OF THE PHARMACOKINETICS AND STABILITY OF ITS RESIDUES [J].

MCCRACKEN, RJ ;

BLANCHFLOWER, WJ ;

ROWAN, C ;

MCCOY, MA ;

KENNEDY, DG .

ANALYST, 1995, 120 (09) :2347-2351

[10] POSTMORTAL DEGRADATION OF FURAZOLIDONE AND FURALTADONE IN EDIBLE TISSUES OF CALVES [J].

NOUWS, JFM ;

LAURENSEN, J .

VETERINARY QUARTERLY, 1990, 12 (01) :56-59

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