Rat ventral prostate microsomal biotransformation of ethanol to acetaldehyde and 1-hydroxyethyl radicals:: Its potential contribution to prostate tumor promotion

被引：10

作者：

Castro, GD ^{[1
]}

de Layño, AMAD ^{[1
]}

Costantini, MH ^{[1
]}

Castro, JA ^{[1
]}

机构：

[1] Consejo Nacl Invest Cient & Tecn, CITEFA, CEITOX, Buenos Aires, DF, Argentina

来源：

TERATOGENESIS CARCINOGENESIS AND MUTAGENESIS | 2002年 / 22卷 / 05期

关键词：

alcohol; spin trapping; free radicals; GC-MS; cancer;

D O I：

10.1002/tcm.10028

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Rat ventral prostate microsomal fraction was able to biotransform ethanol to acetaldehyde and 1-hydroxyethyl radicals (1HEt) in the presence of NADPH and oxygen. The enzymatic processes involved were not inhibited by desferrioxamine, CO, SKF 525A, 4-methylpyrazole, or polyclonal antibody against P450 reductase but they were significantly inhibited by diethyldithiocarbamate, 2-mercapto-1-methylimidazol, thiobenzamide, or diphenyleneiodonium chloride. Results would suggest the partial participation in these ethanol bioactivation processes of flavin containing monooxygenase (FMO) and/or other flavin dependent oxidases/peroxidases and of a non-iron metal-containing enzymes. Acetaldehyde and free radicals production by prostate microsomal fraction might potentially contribute to turner promotion in heavy alcohol drinkers. (C) 2002 Wiley-Liss, Inc.

引用

页码：335 / 341

页数：7

共 30 条

[11]

GAD SC, 1982, PRINCIPLES METHODS T, P273

[12] ALCOHOL AND CANCER [J].

GARRO, AJ ;

LIEBER, CS .

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1990, 30 :219-249

[13]

Gasser R, 1996, EXP TOXICOL PATHOL, V48, P467

[14]

Gómez MID, 2000, TOXICOLOGY, V154, P113

[15]

GUENGUERICH FP, 1995, STRUCTURE MECH BIOCH, P473

[16] USE OF DESFERRIOXAMINE AS A PROBE FOR IRON-DEPENDENT FORMATION OF HYDROXYL RADICALS - EVIDENCE FOR A DIRECT REACTION BETWEEN DESFERAL AND THE SUPEROXIDE RADICAL [J].

HALLIWELL, B .

BIOCHEMICAL PHARMACOLOGY, 1985, 34 (02) :229-233

[17]

Hirayama T., 1992, NATL CANCER I MONOGR, P65

[18]

HODGSON E, 1999, MOL APPL ASPECTS OXI, P55

[19]

Irani K, 1998, BIOCHEM PHARMACOL, V55, P1339

[20]

LIEBER CS, 1996, ALCOHOL GASTROINTEST, P19

← 1 2 3 →