Rpb4 and Rpb9 mediate subpathways of transcription-coupled DNA repair in Saccharomyces cerevisiae

被引：105

作者：

Li, SS ^{[1
]}

Smerdon, MJ ^{[1
]}

机构：

[1] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA

来源：

EMBO JOURNAL | 2002年 / 21卷 / 21期

关键词：

nucleotide excision repair; Rad26; Rpb4; Rpb9; transcription coupled;

D O I：

10.1093/emboj/cdf589

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rpb9, a non-essential subunit of RNA polymerase II, mediates a transcription-coupled repair (TCR) subpathway in Saccharomyces cerevisiae. This subpathway initiates at the same upstream site as the previously identified Rad26 subpathway. However, the Rpb9 subpathway operates more effectively in the coding region than in the region upstream of the transcription start site, whereas the Rad26 subpathway operates equally in the two regions. Rpb4, another non-essential subunit of RNA polymerase II, plays a dual role in regulating the two subpathways, suppressing the Rpb9 subpathway and facilitating the Rad26 subpathway. Simultaneous deletion of RPB9 and RAD26 genes completely abolishes TCR in both the coding and upstream regions, indicating that no other TCR subpathway exists in RNA polymerase II-transcribed genes.

引用

页码：5921 / 5929

页数：9

共 58 条

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