Specification of a DNA replication origin by a transcription complex

被引：123

作者：

Danis, E ^{[1
]}

Brodolin, K ^{[1
]}

Menut, S ^{[1
]}

Maiorano, D ^{[1
]}

Girard-Reydet, C ^{[1
]}

Méchali, M ^{[1
]}

机构：

[1] CNRS, Inst Human Genet, F-34396 Montpellier 5, France

来源：

NATURE CELL BIOLOGY | 2004年 / 6卷 / 08期

关键词：

D O I：

10.1038/ncb1149

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In early Xenopus development, transcription is repressed and DNA replication initiates at non-specific sites. Here, we show that a site-specific DNA replication origin can be induced in this context by the assembly of a transcription domain. Deletion of the promoter element abolishes site-specific initiation, and its relocalization to an ectopic site induces a new origin of replication. This process does not require active transcription, and specification of the origin occurs mainly through a decrease in nonspecific initiation at sites distant from the promoter. Finally, chromatin immunoprecipitation experiments suggest that site-specific acetylation of histones favours the selection of the active DNA replication origin. We propose that the specification of active DNA replication origins occurs by secondary epigenetic events and that the programming of chromatin for transcription during development contributes to this selection in higher eukaryotes.

引用

页码：721 / 730

页数：10

共 47 条

[1] Start sites of bidirectional DNA synthesis at the human lamin B2 origin [J].