Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates

被引：33

作者：

Baldwin, Carson D.

Howe, Gerald B.

Sampath, Ranga ^{[2
]}

Blyn, Larry B. ^{[2
]}

Matthews, Heather ^{[2
]}

Harpin, Vanessa ^{[2
]}

Hall, Thomas A. ^{[2
]}

Drader, Jared J. ^{[2
]}

Hofstadler, Steve A. ^{[2
]}

Eshoo, Mark W. ^{[2
]}

Rudnick, Karl ^{[3
]}

Studarus, Karen ^{[3
]}

Moore, David ^{[3
]}

Abbott, Sharon ^{[4
]}

Janda, J. Michael ^{[4
]}

Whitehouse, Chris A. ^{[1
]}

机构：

[1] USA, Med Res Inst Infect Dis, Diagnost Syst Div, Ft Detrick, MD 21702 USA

[2] Ibis Biosci Inc, Carlsbad, CA 92008 USA

[3] SAIC, San Diego, CA 92121 USA

[4] Calif Dept Publ Hlth, Microbial Dis Lab, Richmond, CA 94804 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2009年 / 63卷 / 04期

关键词：

Broad-range PCR; Mass spectrometry; Rapid detection; Bacterial diagnostics; 16S RIBOSOMAL-RNA; SEQUENCE-ANALYSIS; PCR ASSAY; CLINICAL MICROBIOLOGY; UNIVERSAL BIOSENSOR; IDENTIFICATION; MYCOBACTERIA; AMPLIFICATION; GENES;

D O I：

10.1016/j.diagmicrobio.2008.12.012

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria. Published by Elsevier Inc.

引用

页码：403 / 408

页数：6

共 22 条

[1] Identification of clinical coryneform bacterial isolates:: Comparison of biochemical methods and sequence analysis of 16S rRNA and rpoB genes [J].