Objective: To evaluate the reliability and validity of Stroke Impact Scale 3.0 (SIS 3.0), a quality-of-life scale specific to stroke, in Turkish stroke patients. Material and Methods: One hundred two patients were included in the study. The reliability was assessed by internal consistency (Crohnbach alpha) and test-retest methods (intraclass correlation coefficient (ICCC)). Validity was assessed by correlation between Brunnstrom stages, Mini-Mental State Examination (MMSE), Beck Depression Inventory (BDI), Functional Independence Measure (FIM), Short Form-36 (SF-36), and subgroup and total scores of SIS 3.0. Results: The mean age of patients was 61.5 +/- 12.5 years; 52.0% of the patients were male, and 48.0% were female. For all the subgroups and total score, Crohnbach alpha values were >0.70, and ICCC was 0.947-0.999. With regards to validity, significant positive correlation of the total scores between Brunnstrom stages, MMSE, FIM, and SIS 3.0 and negative correlation between BDI and SIS 3.0 were detected (p<0.05). Except for the pain and general health subgroups (p>0.05), between other subgroups of SF-36 and the total scores of SIS 3.0, a positive significant relationship was found (p<0.05), while there was no correlation between the pain and general health subgroups. Conclusion: The reliability and validity of the Turkish version of SIS 3.0 was shown; hence, we believe that it may be useful in the follow-up of stroke patients and in clinical research.