Cure of human carcinoma xenografts by a single dose of pretargeted yttrium-90 with negligible toxicity

被引：190

作者：

Axworthy, DB ^{[1
]}

Reno, JM ^{[1
]}

Hylarides, MD ^{[1
]}

Mallett, RW ^{[1
]}

Theodore, LJ ^{[1
]}

Gustavson, LM ^{[1
]}

Su, FM ^{[1
]}

Hobson, LJ ^{[1
]}

Beaumier, PL ^{[1
]}

Fritzberg, AR ^{[1
]}

机构：

[1] NeoRx Corp, Seattle, WA 98119 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 04期

关键词：

D O I：

10.1073/pnas.97.4.1802

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A covalent conjugate (NR-LU-10/SA) was prepared between streptavidin (SA) and NR-LU-10, a mAb that binds an antigen expressed on the surface of most human carcinomas. NR-LU-10/SA was injected into nude mice bearing human tumor xenografts. Injection of biotinylated galactosyl-human serum albumin reduced the circulating levels of conjugate by 95%. Subsequent administration of Y-90-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid-biotin achieved peak uptake at the tumor within 2 hr while >80% of the radioactivity was eliminated in the urine. A single dose of 600-800 mu Ci of Y-90-1,4,7,10-tetraazacyclododecane-1.4,7,10-tetraacetic acid-biotin produced cures in 10/10 mice with established (>200 mm(3)) s.c. human small cell lung or colon cancer xenografts and 8/10 cures in mice with human breast cancer xenografts without significant toxicity.

引用

页码：1802 / 1807

页数：6

共 39 条

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