Cytotoxic effects of butanolic extract from Pfaffia paniculata (Brazilian Ginseng) on cultured human breast cancer cell line MCF-7

被引：29

作者：

Nagamine, Marcia Kazumi ^{[1
]}

da Silva, Tereza Cristina ^{[1
]}

Matsuzaki, Patricia ^{[1
]}

Pinello, Katia Cristina ^{[1
]}

Cogliati, Bruno ^{[1
]}

Pizzo, Celia Regina ^{[4
]}

Akisue, Gokithi ^{[2
]}

Haraguchi, Mitsue ^{[3
]}

Gorniak, Silvana Lima ^{[1
]}

Sinhorini, Idercio Luiz ^{[1
]}

Kesava Rao, Kurapati Venkata ^{[1
]}

Marzagao Barbuto, Jose Alexandre ^{[4
]}

Zaidan Dagli, Maria Lucia ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Pathol, Fac Vet Med & Zootechny, BR-05508900 Sao Paulo, Brazil

[2] Univ Sao Francisco, Fac Pharmaceut Sci, BR-12900000 Braganca Paulista, SP, Brazil

[3] Biol Inst Sao Paulo, Pharmacol Sect, Sao Paulo, Brazil

[4] Univ Sao Paulo, Dept Immunol, Inst Biomed Sci, BR-05508900 Sao Paulo, Brazil

来源：

EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY | 2009年 / 61卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

Breast cancer; Cytotoxic effects; MCF-7; cells; Pfaffia paniculata; Plant extracts; NORTRITERPENOID SAPONINS; CHEMOPREVENTION; PHYTOCHEMICALS; PFAFFOSIDES; TUMOR;

D O I：

10.1016/j.etp.2008.01.017

中图分类号：

R36 [病理学];

学科分类号：

100103 [病原生物学];

摘要：

Roots of Pfaffia paniculata have been well documented for multifarious therapeutic values and have also been used for cancer therapy in folk medicine. This study has been performed in a human breast tumor cell line, the MCF-7 cells. These are the most commonly used model of estrogen-positive breast cancer, and it has been originally established in 1973 at the Michigan Cancer Foundation from a pleural effusion taken from a woman with metastatic breast cancer. Butanolic extract of the roots of P. paniculata showed cytotoxic effect MCF-7 cell line. as determined with crystal violet assay, cellular death with acridine orange/ethidium bromide staining, and cell proliferation with immunocytochemistry of bromodeoxyuridine (BrdU). Subcellular alterations were evaluated by electron microscopy. Cells treated With butanolic extract showed degeneration of cytoplasmic components and profound morphological and nuclear alterations. The results show that this butanolic extract indeed presents cytotoxic substances, and its fractions merit further investigations. (C) 2008 Elsevier GmbH. All rights reserved.

引用

页码：75 / 82

页数：8

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