Forskolin induces circadian gene expression of rPer1, rPer2 and dbp in mammalian rat-1 fibroblasts

被引：205

作者：

Yagita, K

Okamura, H

机构：

[1] Kobe Univ, Sch Med, Dept Anat & Brain Sci, Chuo Ku, Kobe, Hyogo 6500017, Japan

[2] Kyoto Prefectural Univ Med, Dept Anat, Kyoto 6028566, Japan

来源：

FEBS LETTERS | 2000年 / 465卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

forskolin; CREB; Per1; Per2; circadian rhythm;

D O I：

10.1016/S0014-5793(99)01724-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammalian culture cells have the potential for periodicity, since high concentrations of serum can elicit the circadian expression of clock genes in rat-1 fibroblasts. However, the mechanism by which serum affects circadian gene expression remains unclear. In the present study, we incubated rat-1 cells with forskolin and successfully induced the rhythmic expression of Per1, Per2 and dhp. In the initial step of the circadian gene expression, a marked transient induction of Per1 was observed accompanied with CREB phosphorylation, Thus the present study strongly suggests that CREB activation through the cAMP/PKA pathway is involved in the generation of circadian rhythm in rat-1 cells (C) 2000 Federation of European Biochemical Societies.

引用

页码：79 / 82

页数：4

共 24 条

[1] A differential response of two putative mammalian circadian regulators, mper1 and mper2, to light [J].