Bixin and norbixin in human plasma: Determination and study of the absorption of a single dose of Annatto Food Color

被引：44

作者：

Levy, LW ^{[1
]}

Regalado, E ^{[1
]}

Navarrete, S ^{[1
]}

Watkins, RH ^{[1
]}

机构：

[1] N CAROLINA STATE UNIV,RALEIGH,NC 27695

来源：

ANALYST | 1997年 / 122卷 / 09期

关键词：

bixin; norbixin; carotenoids; intestinal absorption; human plasma; high-performance liquid chromatography;

D O I：

10.1039/a701304c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A procedure was developed for the detection and determination of bixin and norbixin in human plasma by reversed-phase HPLC with a sensitivity limit of 5 mu g l(-1). A group of seven volunteers ingested a single dose of 1 ml of a commercial Annatto Food Color (16 mg of cis-bixin in soybean oil). The presence of bixin (cis and trans) and norbixin (cis and trans) was demonstrated in the plasma at average levels of 11.6, 10.1, 2.8 and 0 mu g l(-1) of bixin and 48, 58, 53 and 29 mu g l(-1) of norbixin after 2, 4, 6 and 8 h, respectively. Considerable individual variations were observed. Complete plasma clearance generally occurred for bixin by 8 h and for norbixin by 24 h after ingestion of cis-bixin.

引用

页码：977 / 980

页数：4