Insulin-like growth factor I effect on the number of osteoblast progenitors is impaired in ovariectomized mice

被引:22
作者
Kasukawa, Y
Stabnov, L
Miyakoshi, N
Baylink, DJ
Mohan, S
机构
[1] JLP Vet Adm Med Ctr, Musculoskeletal Dis Ctr, Loma Linda, CA 92357 USA
[2] Loma Linda Univ, Dept Med, Loma Linda, CA 92350 USA
[3] Loma Linda Univ, Dept Biochem, Loma Linda, CA 92350 USA
[4] Loma Linda Univ, Dept Physiol, Loma Linda, CA 92350 USA
关键词
insulin-like growth factor I; bone formation; osteoblast progenitors; mice; ovariectomy;
D O I
10.1359/jbmr.2002.17.9.1579
中图分类号
R5 [内科学];
学科分类号
1002 [临床医学]; 100201 [内科学];
摘要
Because insulin-like growth factor (IGF) I is an important regulator of bone formation, we proposed the hypothesis that IGF-I could contribute in regulating the number of osteoblast progenitors (colony-forming unit fibroblast with ALP activity [CFU-F/ALP(+)]). To test ex vivo and in vivo effects of IGF-I on the number of CFU-F/ALP+, bone marrow cells (BMCs) derived from normal mice, growth hormone (GH)-deficient lit/lit mice, or ovariectomized (OVX) mice were cultured and the CFU-F/ALP+ number was counted. Ex vivo treatment of IGF-I increased the CFU-F/ALP+ number in a dose-dependent manner compared with vehicle-treated control cultures. The CFU-F/ALP+ number was decreased by 20% (p < 0.01; n = 7-9) in GH-deficient lit/lit mice compared with age-matched control mice. Four weeks after OVX or sham operation, IGF-I (2 mug/g body wt) or vehicle was administered twice on day 1, and 5 days later, BMCs were removed from the femur and cultured for 10 days (n = 9-10 per group). IGF-I administration increased the CFU-F/ALP+ number by 63% (p < 0.01) and 19% (NS), respectively, in sham-operated (sham) and OVX mice compared with the vehicle-treated control group. The serum IGF-I level was similar in OVX mice compared with sham mice; this finding is different from that found in rats in which OVX increases the serum IGF-I level. This study showed that IGF-I is an important regulator of osteoblast-progenitor number in the BMCs of mice both ex vivo and in vivo and that the IGF-I response to increase the number of osteoblast progenitors was impaired in OVX mice.
引用
收藏
页码:1579 / 1587
页数:9
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