Expression of apoptosis-regulatory genes in epithelial cells in pulmonary fibrosis in mice

被引：12

作者：

Kuwano, K ^{[1
]}

Hagimoto, N ^{[1
]}

Tanaka, T ^{[1
]}

Kawasaki, M ^{[1
]}

Kunitake, R ^{[1
]}

Miyazaki, H ^{[1
]}

Kaneko, Y ^{[1
]}

Matsuba, T ^{[1
]}

Maeyama, T ^{[1
]}

Hara, N ^{[1
]}

机构：

[1] Kyushu Univ, Fac Med, Chest Dis Res Inst, Higashi Ku, Fukuoka 812, Japan

来源：

JOURNAL OF PATHOLOGY | 2000年 / 190卷 / 02期

关键词：

Fas; Fas ligand; p53; p21; bcl-2; bcl-x; bax; apoptosis; bleomycin; pulmonary fibrosis;

D O I：

10.1002/(SICI)1096-9896(200002)190:2<221::AID-PATH495>3.0.CO;2-J

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Up-regulation of Fas and Fas ligand and excessive apoptosis of bronchiolar and alveolar epithelial cells were identified in bleomycin-induced pulmonary fibrosis in mice. This study hypothesized that apoptosis-regulatory genes other than Fas-Fas ligand, such as p53, p21 (Waf1/Cip1), bcl-2, bcl-x, and bax, may also participate in epithelial cell apoptosis in this model. The expression of these genes was assessed by reverse transcription polymerase chain reaction (RT-PCR), RT in situ PCR, or immunohistochemistry. The expression of p53 and p21 mRNA was concurrently upregulated in the alveolar epithelial cells at 1 h to 7 days after intratracheal instillation of bleomycin. The expression of bcl-2 mRNA was weakly up-regulated at 1 h to 14 days, while the expression level of bcl-2 protein was not changed. The expression of bcl-x(L) and bax mRNA was strongly up-regulated at 1 h to 7 days. The expression of bcl-x protein was up-regulated in lymphocytes and macrophages, whereas bax protein was up-regulated in both epithelial and inflammatory cells. It is concluded that epithelial cell apoptosis in this model may also be induced by the up-regulation of p53 and bax and by the imbalance between apoptosis-inducible and -inhibitory genes, in addition to the up-regulation of the Pas-Fas ligand pathway, Copyright (C) 2000 John Wiley & Sons, Ltd.

引用

页码：221 / 229

页数：9

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