Structure of a central component of the yeast kinetochore: The Spc24p/Spc25p globular domain

被引:82
作者
Wei, Ronnie R.
Schnell, Jason R.
Larsen, Nicholas A.
Sorger, Peter K.
Chou, James J.
Harrison, Stephen C. [1 ]
机构
[1] Harvard Univ, Sch Med, Dept Chem Biol & Mol Pharmacol, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA
[3] MIT, Dept Biol, Cambridge, MA 02139 USA
[4] MIT, Biol Engn Div, Cambridge, MA 02139 USA
关键词
D O I
10.1016/j.str.2006.04.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during mitosis. It is an similar to 570 angstrom long, rod-shaped assembly of four proteins-Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p-with globular regions at either end of a central shaft. The complex bridges from the centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2 globular end. We report the atomic structures of the Spc24/Spc25 globular domain, determined both by X-ray crystallography at 1.9 angstrom resolution and by NMR. Spc24 and Spc25 fold tightly together into a single globular entity with pseudo-2-fold symmetry. Conserved residues line a common hydrophobic core and the bottom of a cleft, indicating that the functional orthologs from other eukaryotes will have the same structure and suggesting a docking site for components of the inner kinetochore.
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收藏
页码:1003 / 1009
页数:7
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