Application of the chemical imaging sensor to electrophysiological measurement of a neural cell

被引：24

作者：

Tanaka, H ^{[1
]}

Yoshinobu, T ^{[1
]}

Iwasaki, H ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Ibaraki, Osaka 5670047, Japan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1999年 / 59卷 / 01期

关键词：

sensor; chemical sensor; chemical imaging sensor; neuron; electrophysiology;

D O I：

10.1016/S0925-4005(99)00012-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The chemical imaging sensor is applied to electrophysiological measurement of a neural cell. In this method, neural cells are cultured directly on the sensing surface, and a laser beam is used to specify a cell to be measured. The amplitude of the ac photocurrent induced by the laser beam is measured to detect the change of the membrane potential of the cell. In this study, the membrane potential of the cell is varied by injection of a de current, and it is demonstrated that the ac photocurrent signal responds to the membrane potential. This method would enable noninvasive electric measurement of neural cells, networks or tissues under long-term culture. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：21 / 25

页数：5

共 28 条

[21] OPTICAL RECORDING OF IMPULSES IN INDIVIDUAL NEURONS OF AN INVERTEBRATE CENTRAL NERVOUS-SYSTEM [J].

SALZBERG, BM ;

DAVILA, HV ;

COHEN, LB .

NATURE, 1973, 246 (5434) :508-509

[22] CAP-PCR Useful for Analyzing the Kin Relatedness of a Small Ant Species [J].

T. Murakami ;

Y. Ishibashi ;

S. Abe ;

M. C. Yoshida ;

S Higashi .

Naturwissenschaften, 1997, 84 (2) :86-88

[23] INTEGRATED BIOSENSOR EMPLOYING A SURFACE PHOTOVOLTAGE TECHNIQUE [J].

SHIMIZU, M ;

KANAI, Y ;

UCHIDA, H ;

KATSUBE, T .

SENSORS AND ACTUATORS B-CHEMICAL, 1994, 20 (2-3) :187-192

[24]

SUGIHARA H, 1996, NATL TECH REP, V42, P260

[25] High speed chemical image sensor with digital LAPS system [J].

Uchida, H ;

Zhang, WY ;

Katsube, T .

SENSORS AND ACTUATORS B-CHEMICAL, 1996, 34 (1-3) :446-449

[26] Visualization of chemical waves in Belousav-Zhabotinsky reaction by chemical imaging sensors [J].

Yoshinobu, T ;

Oba, N ;

Iwasaki, H .

JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 1997, 144 (11) :3919-3921

[27] Application of chemical imaging sensor to electro generated pH distribution [J].

Yoshinobu, T ;

Iwasaki, H ;

Nakao, M ;

Nomura, S ;

Nakanishi, T ;

Takamatsu, S ;

Tomita, K .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS, 1998, 37 (3B) :L353-L355

[28]

Yoshinobu T., 1996, Sensors and Actuators A (Physical), VA51, P231, DOI 10.1016/0924-4247(96)01226-5

← 1 2 3 →