Identification of macromolecular complexes in cryoelectron tomograms of phantom cells

被引:198
作者
Frangakis, AS [1 ]
Böhm, J [1 ]
Förster, F [1 ]
Nickell, S [1 ]
Nicastro, D [1 ]
Typke, D [1 ]
Hegerl, R [1 ]
Baumeister, W [1 ]
机构
[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany
关键词
D O I
10.1073/pnas.172520299
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Electron tomograms of intact frozen-hydrated cells are essentially three-dimensional images of the entire proteome of the cell, and they depict the whole network of macromolecular interactions. However, this information is not easily accessible because of the poor signal-to-noise ratio of the tomograms and the crowded nature of the cytoplasm. Here, we describe a template matching algorithm that is capable of detecting and identifying macromolecules in tomographic volumes in a fully automated manner. The algorithm is based on nonlinear cross correlation and incorporates elements of multivariate statistical analysis. Phantom cells, i.e., lipid vesicles filled with macromolecules, provide a realistic experimental scenario for an assessment of the fidelity of this approach. At the current resolution of approximate to4 nm, macromolecules in the size range of 0.5-1 MDa can be identified with good fidelity.
引用
收藏
页码:14153 / 14158
页数:6
相关论文
共 14 条
  • [1] The cell as a collection of protein machines: Preparing the next generation of molecular biologists
    Alberts, B
    [J]. CELL, 1998, 92 (03) : 291 - 294
  • [2] Toward detecting and identifying macromolecules in a cellular context:: Template matching applied to electron tomograms
    Böhm, J
    Frangakis, AS
    Hegerl, R
    Nickell, S
    Typke, D
    Baumeister, W
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (26) : 14245 - 14250
  • [3] CRYO-ELECTRON MICROSCOPY OF VITRIFIED SPECIMENS
    DUBOCHET, J
    ADRIAN, M
    CHANG, JJ
    HOMO, JC
    LEPAULT, J
    MCDOWALL, AW
    SCHULTZ, P
    [J]. QUARTERLY REVIEWS OF BIOPHYSICS, 1988, 21 (02) : 129 - 228
  • [4] Macromolecular crowding: obvious but underappreciated
    Ellis, RJ
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (10) : 597 - 604
  • [5] Segmentation of two- and three-dimensional data from electron microscopy using eigenvector analysis
    Frangakis, AS
    Hegerl, R
    [J]. JOURNAL OF STRUCTURAL BIOLOGY, 2002, 138 (1-2) : 105 - 113
  • [6] Wavelet transform filtering and nonlinear anisotropic diffusion assessed for signal reconstruction performance on multidimensional biomedical data
    Frangakis, AS
    Stoschek, A
    Hegerl, R
    [J]. IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2001, 48 (02) : 213 - 222
  • [7] Determination of the inelastic mean free path in ice by examination of tilted vesicles and automated most probable loss imaging
    Grimm, R
    Typke, D
    Barmann, M
    Baumeister, W
    [J]. ULTRAMICROSCOPY, 1996, 63 (3-4) : 169 - 179
  • [8] ATPase cycle of an archaeal chaperonin
    Gutsche, I
    Mihalache, O
    Baumeister, W
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2000, 300 (01) : 187 - 196
  • [9] The EM program package: A platform for image processing in biological electron microscopy
    Hegerl, R
    [J]. JOURNAL OF STRUCTURAL BIOLOGY, 1996, 116 (01) : 30 - 34
  • [10] Perspectives of molecular and cellular electron tomography
    Koster, AJ
    Grimm, R
    Typke, D
    Hegerl, R
    Stoschek, A
    Walz, J
    Baumeister, W
    [J]. JOURNAL OF STRUCTURAL BIOLOGY, 1997, 120 (03) : 276 - 308