Nuclear pseudogenes of mitochondrial DNA as a variable part of the human genome

被引：16

作者：

Yuan, JD

Shi, JX

Meng, GX

An, LG

Hu, GX

机构：

[1] Chinese Acad Sci, Shanghai Inst Cell Biol, Shanghai 200031, Peoples R China

[2] Chinese Acad Sci, Shanghai Life Sci Ctr, Shanghai 200031, Peoples R China

[3] Shandong Normal Univ, Dept Biol, Jinan 250014, Peoples R China

来源：

CELL RESEARCH | 1999年 / 9卷 / 04期

关键词：

gene amplification; genome instability; mitochondrial; pseudogene;

D O I：

10.1038/sj.cr.7290027

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Novel pseudogenes homologous to the mitochondrial (mt) 16S rRNA gene were detected via different approaches. Eight pseudogenes were sequenced. Copy number polymorphism of the mtDNA pseudogenes was observed among randomly chosen individuals, and even among siblings. A mtDNA pseudogene in the Y-chromosome was observed in a YAC clone carrying only repetitive sequence tag site (STS). PCR screening of human yeast artificial chromosome (YAC) libraries showed that there were at least 5.7 x 10(5) bp of the mtDNA pseudogenes in each haploid nuclear genome. Possible involvement of the mtDNA pseudogenes in the variable part of the human nuclear genome is discussed.

引用

收藏

页码：281 / 290

页数：10

相关论文

共 40 条

[1]

Agsteribbe E, 1982, NATURE, V298, P187

[2] CONSTRUCTION AND CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME LIBRARY CONTAINING 7 HAPLOID HUMAN GENOME EQUIVALENTS [J].

ALBERTSEN, HM ;

ABDERRAHIM, H ;

CANN, HM ;

DAUSSET, J ;

LEPASLIER, D ;

COHEN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) :4256-4260

[3] SEQUENCE AND ORGANIZATION OF THE HUMAN MITOCHONDRIAL GENOME [J].

ANDERSON, S ;

BANKIER, AT ;

BARRELL, BG ;

DEBRUIJN, MHL ;

COULSON, AR ;

DROUIN, J ;

EPERON, IC ;

NIERLICH, DP ;

ROE, BA ;

SANGER, F ;

SCHREIER, PH ;

SMITH, AJH ;

STADEN, R ;

YOUNG, IG .

NATURE, 1981, 290 (5806) :457-465

[4] DETERMINATION OF GENE DOSAGE BY A QUANTITATIVE ADAPTATION OF THE POLYMERASE CHAIN-REACTION (GD-PCR) - RAPID DETECTION OF DELETIONS AND DUPLICATIONS OF GENE-SEQUENCES [J].

CELI, FS ;

COHEN, MM ;

ANTONARAKIS, SE ;

WERTHEIMER, E ;

ROTH, J ;

SHULDINER, AR .

GENOMICS, 1994, 21 (02) :304-310

[5] QUANTITATIVE PCR - VALIDATION OF THE USE OF A MULTISPECIFIC INTERNAL CONTROL [J].

COTTREZ, F ;

AURIAULT, C ;

CAPRON, A ;

GROUX, H .

NUCLEIC ACIDS RESEARCH, 1994, 22 (13) :2712-2713

[6] EXPRESSION OF MALIGNANCY IN HYBRIDS BETWEEN NORMAL AND MALIGNANT-CELLS [J].

CROCE, CM ;

BARRICK, J ;

LINNENBACH, A ;

KOPROWSKI, H .

JOURNAL OF CELLULAR PHYSIOLOGY, 1979, 99 (03) :279-285

[7] REARRANGED MITOCHONDRIAL GENES IN THE YEAST NUCLEAR GENOME [J].

FARRELLY, F ;

BUTOW, RA .

NATURE, 1983, 301 (5898) :296-301

[8] THE HUMAN Y-CHROMOSOME - OVERLAPPING DNA CLONES SPANNING THE EUCHROMATIC REGION [J].

FOOTE, S ;

VOLLRATH, D ;

HILTON, A ;

PAGE, DC .

SCIENCE, 1992, 258 (5079) :60-66

[9] MITOCHONDRIAL DNA-LIKE SEQUENCES IN THE HUMAN NUCLEAR GENOME - CHARACTERIZATION AND IMPLICATIONS IN THE EVOLUTION OF MITOCHONDRIAL-DNA [J].

FUKUDA, M ;

WAKASUGI, S ;

TSUZUKI, T ;

NOMIYAMA, H ;

SHIMADA, K ;

MIYATA, T .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 186 (02) :257-266

[10] MITOCHONDRIAL-DNA SEQUENCES IN THE NUCLEAR GENOME OF A LOCUST [J].

GELLISSEN, G ;

BRADFIELD, JY ;

WHITE, BN ;

WYATT, GR .

NATURE, 1983, 301 (5901) :631-634

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