A human cDNA sequence with homology to non-mammalian lysophosphatidic acid acyltransferases

被引：41

作者：

Stamps, AC

Elmore, MA

Hill, ME

Kelly, K

Makda, AA

Finnen, MJ

机构：

[1] Yamanouchi Research Institute, Littlemore, Oxford 0X4 4SX, Littlemore Park

来源：

BIOCHEMICAL JOURNAL | 1997年 / 326卷

关键词：

D O I：

10.1042/bj3260455

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel human homologue of Escherichia coli, yeast and plant 1-acylglycerol-3-phosphate acyltransferase has been isolated from U937 cell cDNA. Expression of the cloned sequence in 1-acylglycerol-3-phosphate acyltransferase-deficient E. coli resulted in increased incorporation of oleic acid into cellular phospholipids. Membranes made from COS7 cells transfected with the cDNA exhibited higher acyltransferase activity towards a range of donor fatty acyl-CoAs and lysophosphatidic acid. Northern-blot analysis of the cDNA sequence indicated high levels of expression in immune cells and epithelium. Rapid amplification of cDNA ends revealed differentially expressed splice variants, which suggests regulation of the enzyme by alternative splicing. This cDNA therefore represents the first described sequence of a mammalian gene homologous to non-mammalian lysophosphatidic acid acyltransferases.

引用

页码：455 / 461

页数：7

共 24 条

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