Factors influencing regeneration of soybean from mature and immature cotyledons

被引：33

作者：

Franklin, G ^{[1
]}

Carpenter, L ^{[1
]}

Davis, E ^{[1
]}

Reddy, CS ^{[1
]}

Al-Abed, D ^{[1
]}

Abou Alaiwi, W ^{[1
]}

Parani, M ^{[1
]}

Smith, B ^{[1
]}

Goldman, SL ^{[1
]}

Sairam, RV ^{[1
]}

机构：

[1] Univ Toledo, Plant Sci Res Ctr, Toledo, OH 43606 USA

来源：

PLANT GROWTH REGULATION | 2004年 / 43卷 / 01期

关键词：

cotyledon; Glycine max; organogenic nodule; shoot regeneration; soybean;

D O I：

10.1023/B:GROW.0000038359.86756.18

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Soybean (Glycine max L.) plantlets were efficiently regenerated from mature and immature cotyledons of five different genotypes by studying various parameters affecting regeneration. Green organogenic nodules were induced at the proximal end, which subsequently differentiated into shoot buds on modified MS (Murashige T. and Skoog F. 1962. Physiol. Plant. 15: 473-497) medium. The presence of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) in the medium exerted a synergistic effect, in that regeneration efficiency was higher than for either cytokin alone. The regenerated shoot buds elongated and rooted on MS medium containing 0.29 muM gibberellic acid (GA(3)) and 2.69 muM alpha-naphthaleneaceticacid (NAA), respectively. Rooted plants were established in the greenhouse with 87% success and produced viable seeds. Preliminary studies with Agrobacterium show great promise for soybean transformation based on the regeneration protocol reported here.

引用

页码：73 / 79

页数：7

共 29 条

[1] Appropriate choice of antibiotic and Agrobacterium strain improves transformation of anti biotic-sensitive Fragaria vesca and F-v. semperflorens [J].