Arabidopsis DAYU/ABERRANT PEROXISOME MORPHOLOGY9 Is a Key Regulator of Peroxisome Biogenesis and Plays Critical Roles during Pollen Maturation and Germination in Planta

被引:37
作者
Li, Xin-Ran [1 ,2 ]
Li, Hong-Ju [1 ]
Yuan, Li [1 ]
Liu, Man [1 ]
Shi, Dong-Qiao [1 ]
Liu, Jie [1 ]
Yang, Wei-Cai [1 ]
机构
[1] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
基金
美国国家科学基金会;
关键词
JASMONIC ACID BIOSYNTHESIS; STORAGE ORGANELLE FORMATION; MALE-STERILE MUTANT; PROTEIN IMPORT; ENDOPLASMIC-RETICULUM; PISTIL INTERACTIONS; ANTHER DEHISCENCE; UBIQUITIN LIGASES; TETRAD ANALYSIS; GENE ENCODES;
D O I
10.1105/tpc.113.121087
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pollen undergo a maturation process to sustain pollen viability and prepare them for germination. Molecular mechanisms controlling these processes remain largely unknown. Here, we report an Arabidopsis thaliana mutant, dayu (dau), which impairs pollen maturation and in vivo germination. Molecular analysis indicated that DAU encodes the peroxisomal membrane protein ABERRANT PEROXISOME MORPHOLOGY9 (APEM9). DAU is transiently expressed from bicellular pollen to mature pollen during male gametogenesis. DAU interacts with peroxisomal membrane proteins PEROXIN13 (PEX13) and PEX16 in planta. Consistently, both peroxisome biogenesis and peroxisome protein import are impaired in dau pollen. In addition, the jasmonic acid (JA) level is significantly decreased in dau pollen, and the dau mutant phenotype is partially rescued by exogenous application of JA, indicating that the male sterility is mainly due to JA deficiency. In addition, the phenotypic survey of peroxin mutants indicates that the PEXs most likely play different roles in pollen germination. Taken together, these data indicate that DAU/APEM9 plays critical roles in peroxisome biogenesis and function, which is essential for JA production and pollen maturation and germination.
引用
收藏
页码:619 / 635
页数:17
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