A stable line of transgenic medaka (Oryzias latipes) carrying the CAT gene

A stable homozygous line of transgenic medaka (Oryzias latipes) was produced by injecting plasmid DNA containing rainbow trout metallothionein A promoter region followed by bacterial acetyltransferase gene (rtMT-A-CAT), into the cytoplasm of 111 fertilized eggs. The line transmitted active CAT gene to all of the offsprings until sixth generation in mendelian fashion, The Southern blot analysis and the crossing experiments indicated that the DNA was integrated into the chromosome, These results reveal that the medaka is a good model for basic studies of the production of transgenic fish.