共 22 条
RNA polymerase activity catalyzed by a potyvirus-encoded RNA-dependent RNA polymerase
被引:82
作者:
Hong, YL
[1
]
Hunt, AG
[1
]
机构:
[1] UNIV KENTUCKY, DEPT AGRON, AGR SCI CTR N N212A, LEXINGTON, KY 40546 USA
来源:
关键词:
D O I:
10.1006/viro.1996.0639
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
We have expressed the putative RNA-dependent RNA polymerase encoded by the potyvirus tobacco vein mottling virus (TVMV) in Escherichia coli as a glutathione S-transferase fusion protein. As prepared, the fusion protein possessed the poly(U) polymerase activity that is a hallmark of other picornavirus-encoded polymerases. In addition, this protein was able to utilize full-length TVMV RNA as a template for RNA synthesis. A fusion protein containing a mutation in the highly conserved GDD motif of the polymerase (GDD --> ADD) possessed 7% of the activity of the wild type. Our results confirm that the presumed polymerase encoded by TVMV is in fact an RNA-dependent RNA polymerase and that the GDD motif so widely seen in viral polymerases has an important function in the TVMV protein. (C) 1996 Academic Press, Inc.
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页码:146 / 151
页数:6
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