In-vivo imaging of murine tumors using complete-angle projection fluorescence molecular tomography

被引：37

作者：

Deliolanis, Nikolaos C. ^{[1
,2
]}

Dunham, Joshua ^{[2
]}

Wurdinger, Thomas ^{[2
,3
]}

Figueiredo, Jose-Luiz ^{[2
]}

Bakhos A., Tannous ^{[2
,3
]}

Ntziachristos, Vasilis ^{[1
,2
]}

机构：

[1] Tech Univ Munich, Helmholtz Zentrum Munchen, Inst Biol & Med Imaging, D-85764 Neuherberg, Germany

[2] Harvard Univ, Massachusetts Gen Hosp, Ctr Mol Imaging Res, Charlestown, MA 02129 USA

[3] Harvard Univ, Massachusetts Gen Hosp, Mol Neurogenet Unit, Charlestown, MA 02129 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2009年 / 14卷 / 03期

基金：

美国国家卫生研究院;

关键词：

fluorescence; tomography; in vivo; molecular imaging; cancer; tumor; fluorescent proteins; DIFFUSE OPTICAL TOMOGRAPHY; BREAST-CANCER; RECONSTRUCTION;

D O I：

10.1117/1.3149854

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We interrogate the ability of free-space fluorescence tomography to image small animals in vivo using charge-coupled device (CCD) camera measurements over 360-deg noncontact projections. We demonstrate the performance of normalized dual-wavelength measurements that are essential for in-vivo use, as they account for the heterogeneous distribution of photons in tissue. In-vivo imaging is then showcased on mouse lung and brain tumors cross-validated by x-ray microcomputed tomography and histology. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3149854]

引用

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