Ubiquitin conjugate dynamics in the gut and liver of hibernating ground squirrels

被引:35
作者
vanBreukelen, F
Carey, HV
机构
[1] Univ Wisconsin, Sch Vet Med, Dept Comparat Biosci, Madison, WI 53706 USA
[2] Univ Colorado, Sch Med, Dept Cellular & Struct Biol, Denver, CO 80262 USA
来源
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY | 2002年 / 172卷 / 03期
基金
美国国家科学基金会;
关键词
protein degradation; proteolysis; stress; hibernation;
D O I
10.1007/s00360-002-0252-5
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Protein synthesis is severely depressed in hibernating mammals. In the absence of significant protein synthesis, the continued turnover of proteins as a function of normal cellular activity would result in the net depletion of protein pools. We measured levels of ubiquitylated proteins in the gut of thirteen-lined ground squirrels (Spermophilus tridecemlineatus) and liver of golden-mantled ground squirrels (Spermophilus lateralis). In both tissues, ubiquitin conjugate concentrations increased during entrance into torpor and were elevated 2-3 fold by late torpor compared with levels in active animals. The data are consistent with a depression of proteolysis with a resultant high level of ubiquitylated proteins during the natural hypothermia of torpor. The periodic returns to euthermy during the hibernation season allow for degradation of these conjugated proteins and may serve to restore protein pools.
引用
收藏
页码:269 / 273
页数:5
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