Rapid analysis of triterpenic acids by liquid chromatography using porous graphitic carbon and evaporative light scattering detection

被引：32

作者：

Bérangère, C ^{[1
]}

Caussarieu, N ^{[1
]}

Morin, P ^{[1
]}

Morin-Allory, L ^{[1
]}

Lafrosse, M ^{[1
]}

机构：

[1] Univ Orleans, ICOA, UFR Sci, UMR 6005,CNRS, F-45067 Orleans 2, France

来源：

JOURNAL OF SEPARATION SCIENCE | 2004年 / 27卷 / 12期

关键词：

betulinic acid; oleanolic acid; ursolic acid; glycyrrhetinic acid; liquid chromatography; porous graphitic carbon; evaporative light scattering detector; quantitative structure retention relationship;

D O I：

10.1002/jssc.200401764

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

An original system which uses Porous Graphitic Carbon as support and a mixture of organic solvents as mobile phase is proposed for the analysis of triterpenic acids by liquid chromatography. The separation of betulinic acid, ursolic acid, oleanolic acid, and 18alpha- and 18beta-glycyrrhetinic acids was carried out within a short time and monitored by evaporative light scattering detection as universal detection method. Molecular modelling studies show that the main contribution to the selectivity comes from the electrostatic interaction characterised by the dipole moment of the products.

引用

页码：964 / 970

页数：7

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