mraW, an essential gene at the dcw cluster of Escherichia coli codes for a cytoplasmic protein with methyltransferase activity

被引：24

作者：

Carrión, M ^{[1
]}

Gómez, MJ ^{[1
]}

Merchante-Schubert, R ^{[1
]}

Dongarrá, S ^{[1
]}

Ayala, JA ^{[1
]}

机构：

[1] CSIC UAM, Ctr Biol Mol Severo Ochoa, Madrid 28049, Spain

来源：

BIOCHIMIE | 1999年 / 81卷 / 8-9期

关键词：

methyltransferase; MraW protein; dcw cluster; cell division; Escherichia coli;

D O I：

10.1016/S0300-9084(99)00208-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three new open reading frames, mraZ, mraW and mraR (also called ftsL), were revealed by DNA sequencing immediately upstream of gene pbpB in the dcw cluster of Escherichia coli. We have found that mraW and mraZ are active genes, coding for two proteins with relative molecular masses of 34 800 and 17 300, respectively. MraW is a cytoplasmic protein that under overproduction condition is also loosely bound to the membrane. Soluble MraW was purified up to 90% by a single high performance electrophoresis (HPEC) step from an extract of an overproducing strain. The protein exhibits a S-adenosyl-dependent methyltransferase activity on membrane-located substrates. (C) 1999 Societe francaise de biochimie et biologie moleculaire/Editions scientifiques et medicales Elsevier SAS.

引用

页码：879 / 888

页数：10

共 42 条

[1] The bimodular G57-V577 polypeptide chain of the class B penicillin-binding protein 3 of Escherichia coli catalyzes peptide bond formation from thiolesters and does not catalyze glycan chain polymerization from the lipid II intermediate [J].

Adam, M ;

Fraipont, C ;

Rhazi, N ;

NguyenDisteche, M ;

Lakaye, B ;

Frere, JM ;

Devreese, B ;

VanBeeumen, J ;

vanHeijenoort, Y ;

vanHeijenoort, J ;

Ghuysen, JM .

JOURNAL OF BACTERIOLOGY, 1997, 179 (19) :6005-6009

[2] FtsN, a late recruit to the septum in Escherichia coli [J].

Addinall, SG ;

Cao, C ;

Lutkenhaus, J .

MOLECULAR MICROBIOLOGY, 1997, 25 (02) :303-309

[3]

Addinall Stephen G., 1996, Journal of Bacteriology, V178, P7167

[4] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[5] SITE-DIRECTED MUTAGENESIS OF PENICILLIN-BINDING PROTEIN-3 OF ESCHERICHIA-COLI - ROLE OF VAL-545 [J].

AYALA, J ;

GOFFIN, C ;

NGUYENDISTECHE, M ;

GHUYSEN, JM .

FEMS MICROBIOLOGY LETTERS, 1994, 121 (02) :251-256

[6]

Ayala JA., 1994, BACTERIAL CELL WALL, P73

[7] FTSZ AND CELL-DIVISION [J].

BI, E ;

DAI, K ;

SUBBARAO, S ;

BEALL, B ;

LUTKENHAUS, J .

RESEARCH IN MICROBIOLOGY, 1991, 142 (2-3) :249-252

[8] FTSZ RING STRUCTURE ASSOCIATED WITH DIVISION IN ESCHERICHIA-COLI [J].

BI, E ;

LUTKENHAUS, J .

NATURE, 1991, 354 (6349) :161-164

[9] EVIDENCE FOR INVOLVEMENT OF PENICILLIN-BINDING PROTEIN-3 IN MUREIN SYNTHESIS DURING SEPTATION BUT NOT DURING CELL ELONGATION [J].

BOTTA, GA ;

PARK, JT .

JOURNAL OF BACTERIOLOGY, 1981, 145 (01) :333-340

[10]

Buddelmeijer N, 1998, J BACTERIOL, V180, P6107

← 1 2 3 4 5 →